Myosin II activity dependent and independent vinculin recruitment to the sites of E-cadherin-mediated cell-cell adhesion
© Sumida et al; licensee BioMed Central Ltd. 2011
Received: 6 August 2011
Accepted: 3 November 2011
Published: 3 November 2011
Maintaining proper adhesion between neighboring cells depends on the ability of cells to mechanically respond to tension at cell-cell junctions through the actin cytoskeleton. Thus, identifying the molecules involved in responding to cell tension would provide insight into the maintenance, regulation, and breakdown of cell-cell junctions during various biological processes. Vinculin, an actin-binding protein that associates with the cadherin complex, is recruited to cell-cell contacts under increased tension in a myosin II-dependent manner. However, the precise role of vinculin at force-bearing cell-cell junctions and how myosin II activity alters the recruitment of vinculin at quiescent cell-cell contacts have not been demonstrated.
We generated vinculin knockdown cells using shRNA specific to vinculin and MDCK epithelial cells. These vinculin-deficient MDCK cells form smaller cell clusters in a suspension than wild-type cells. In wound healing assays, GFP-vinculin accumulated at cell-cell junctions along the wound edge while vinculin-deficient cells displayed a slower wound closure rate compared to vinculin-expressing cells. In the presence of blebbistatin (myosin II inhibitor), vinculin localization at quiescent cell-cell contacts was unaffected while in the presence of jasplakinolide (F-actin stabilizer), vinculin recruitment increased in mature MDCK cell monolayers.
These results demonstrate that vinculin plays an active role at adherens junctions under increased tension at cell-cell contacts where vinculin recruitment occurs in a myosin II activity-dependent manner, whereas vinculin recruitment to the quiescent cell-cell junctions depends on F-actin stabilization.
Cells experience force and, therefore, need to mechanically respond to stabilize cell junctions with both neighboring cells and the underlying extracellular matrix. Cadherins are the adhesion proteins composing the adherens junctions at cell-cell contacts while cadherin-associated proteins and the actin cytoskeleton provide stability and structural support between neighboring cells. The E-cadherin complex was identified as a mechanosensor at cell-cell contacts where applying force on the extracellular domain of E-cadherin resulted in vinculin-dependent cell stiffening . Vinculin-dependent cell stiffening was also observed at integrin junctions [2, 3], suggesting a similar role for vinculin at both force-bearing cell-cell and cell-matrix junctions. Thus, vinculin may be a key protein in generating tension at cell-cell contacts in response to external forces from neighboring cells.
Vinculin was originally identified as a protein associated at the ends of actin fibers terminating at the plasma membrane . Along with F-actin [5, 6], additional binding partners to vinculin at focal adhesions include talin [7, 8], paxillin , α-actinin , and phospholipids [11, 12]. Vinculin is composed of a head and tail domain that is linked together by a proline-rich linker region and exists in either an open, activated state or a closed, auto-inhibited state where the head and tail domains interact [13–15]. In the open state, previously hidden sites for vinculin binding partners are exposed. Vinculin activation is achieved through interacting with one of several vinculin binding partners [14, 16–18].
The association of vinculin with integrins at focal adhesions has been well examined, where vinculin binds to talin and paxillin, two integrin-binding proteins [7–9]. At focal adhesions, vinculin is involved in mechano-coupling between the integrins bound to the underlying extracellular matrix, and the actin cytoskeleton [3, 19, 20]. In this position, vinculin plays a major role in force-generating processes such as cell migration on a two-dimensional surface  and cell invasion in a three-dimensional matrix . Vinculin regulates actomyosin force generation in response to external cues through the vinculin tail domain . Although vinculin at focal adhesions has been well studied, the role of vinculin at cell-cell contacts has not.
In biochemical assays with purified proteins, a direct interaction occurs between the vinculin head domain and the cadherin-associated protein α-catenin [23–25], with the vinculin binding site on α-catenin located between aa 326-509 [14, 24]. The additional vinculin interactions with β-catenin [26, 27] or myosin VI  have also been reported. Interestingly, vinculin recruitment to cell-cell contacts is decreased by the myosin II inhibitor blebbistatin in some epithelial cell lines [1, 25, 29], thus supporting the role of vinculin in responding to increased tension at cell-cell contacts. An inhibitory region for vinculin binding was identified on α-catenin (aa 510-697) and suggested α-catenin existing in either a closed conformation with the inhibitory domain occluding the vinculin binding site, or in an open conformation under increased tension with the vinculin binding site exposed .
MDCK cells are the prototypical polarized epithelial cell model, yet the interaction of vinculin with the E-cadherin complex in MDCK cells is different from other cell lines. This has been attributed to low tension in MDCK cell monolayers under normal conditions possibly due to the unique cadherin distribution of MDCK cell-cell contacts , thus resulting in the lack of zonula adherens with vinculin accumulation that is observed in other epithelial cell lines . However, vinculin accumulation increases at adherens junctions lining the wound edge in the MDCK wound-healing model, a process inhibited by blebbistatin [25, 29]. Additionally, an α-catenin antibody recognizing a sequence near the vinculin binding site was localized at the junctions along the wound edge, therefore, indicating the availability of vinculin binding sites on α-catenin at cell-cell contacts under increased tension . Also, in HGF-treated highly migratory MDCK cells, increased vinculin recruitment to cell-cell contacts is reversed with the inclusion of blebbistatin, suggesting myosin II-dependence . This indicates that vinculin is recruited under conditions of increased tension to cell-cell contacts in a myosin II activity-dependent manner. Interestingly, in the MDCK cell monolayer with low tension cell-cell contacts, vinculin still localizes to cell-cell contacts. Therefore, additional factors (myosin II-independent) may be recruiting vinculin to sites of cell-cell contacts, but the mechanisms by which vinculin accumulates under these conditions remain unclear.
The aim of this study was to investigate the role of vinculin at force-bearing adherens junctions and its recruitment to sites of cell-cell contact. In this report, using vinculin knockdown and GFP-vinculin expressing cells in wound-healing assays, we demonstrate that vinculin is required for proper wound closure and vinculin-deficiency results in a reduced rate of wound closure. Additionally, we show that recruitment of vinculin to cell-cell contacts is increased with F-actin stabilization.
Results and Discussion
Generation of vinculin-deficient cells
To further analyze the relative distribution of vinculin, we immuno-labeled vinculin in wild-type and vinculin knockdown cells. The immuno-labeled vinculin localized to the cytoplasm and focal adhesions in wild-type cells, but significantly less in knockdown cells (Figure 1C and 1D). Preferential vinculin localization to focal adhesions in both knockdown cells (Figure 1C and 1D), albeit less intense than wild-type cells, is consistent with the increased insoluble vinculin pool observed with biochemical analysis (Figure 1A). In a confluent MDCK monolayer, vinculin localized to cell-cell contacts in both wild-type and knockdown cells (Figure 1C), but the vinculin intensity at cell-cell contacts of knockdown cells was much weaker (Figure 1C and 1E). While comparable vinculin levels were present at the focal adhesions between the two knockdown cell lines (Figure 1D), KD2 had significantly less vinculin at cell-cell contacts compared to KD1 (Figure 1E), consistent with KD2 having a lower level of vinculin than KD1 (Figure 1A and 1B). Together, in vinculin knockdown cells, the residual vinculin preferentially localized to focal adhesions and cell-cell contacts while depleting most of the cytoplasmic vinculin pool.
The vinculin knockdown cells have weaker cell-cell adhesion
Vinculin is required for efficient wound healing
Despite the reduced vinculin level at cell-cell contacts, vinculin knockdown cells form a cell monolayer without any phenotypic defects (Figure 1C). Since vinculin has been shown to localize to force-bearing sites of wound closing cells, we analyzed collective cell movement during wound healing by creating a single cell-sized opening in a mature monolayer. In this wound healing model, formation and contraction of the actomyosin purse-string surrounding the closing wound generates tension on adherens junctions at cell-cell contacts along the wound edge [29, 31]. In addition, vinculin was previously observed as punctate formations at cell-cell contacts along the wound edge, concurrent with increased adherens junctions formation .
Myosin II independent vinculin accumulation at cell-cell contacts
Calyculin-A is a serine/threonine phosphatase inhibitor that inhibits Myosin Light Chain (MLC) phosphatase. The inhibition of MLC phosphatase is thought to induce myosin II activity by minimizing the de-phosphorylation of MLC, thus myosin II remains in a phosphorylated and active state. In the presence of calyculin-A, numerous actin bundles formed at the basal surface (Figure 4B), a sign of increased myosin II-dependent contractility by calyculin A. Interestingly, the intensity of vinculin at cell-cell contacts was unchanged (Figure 4A). This observation suggests that a mere increase in myosin II activation does not recruit vinculin to cell-cell contacts of MDCK cells. Although myosin II deactivation is sufficient for the vinculin dissociation from cell-cell contacts in some cells, the vinculin recruitment to cell-cell contacts may require additional factors.
Actin stabilization recruits vinculin to cell-cell contacts
To test whether stabilizing F-actin affects vinculin recruitment to cell-cell contacts under tension, jasplakinolide treatment was included in the MDCK wound-healing assay. In the presence of jasplakinolide, vinculin accumulated at cell-cell contacts along the wound edge similarly to control (Figure 5C). Although there was no significant difference in initial wound-healing rates between control and jasplakinolide-treated cells, a trend towards jasplakinolide decreasing initial velocity was observed (Figure 5D). This suggests that actin stabilization reduces the wound closure rate, and that actin dynamics is required for efficient wound closure.
On the other hand, vinculin accumulation was either reduced or absent along the wound edge in blebbistatin-treated cells (Figure 5C), likely due to the lack of contractile actin network. Consistent with a previous study , these cells failed to close the wound, suggesting that myosin II activity is required for wound closure. Since jasplakinolide treatment did not change vinculin recruitment to the wound edge, the vinculin recruitment is solely dependent on myosin II activity. This is in contrast to a mature cell monolayer, where vinculin recruitment requires F-actin stabilization.
Our data suggests the existence of two vinculin pools at the sites of cell-cell adhesion: myosin II dependent and independent pools. In MDCK cells, the myosin II dependent pool is revealed only when cell-cell contacts are under high tension (e.g. force-bearing contacts during wound healing, Figure 5C), while at quiescent cell-cell contacts, vinculin recruitment is enhanced by pharmacologically stabilized F-actin (Figure 5A and 5B). Note that, at quiescent cell-cell contacts, both blebbistatin (Figure 4A) and jasplakinolide treatments (Figure 5A and 5B) increased F-actin intensity, but, only F-actin stabilization with jasplakinolide increased vinculin recruitment at cell-cell contacts, suggesting that the F-actin level does not always correlate with the vinculin recruitment. The recruitment of the contractility-dependent vinculin pool is likely mediated by force-sensitive conformational change of α-catenin, which in turn exposes a previously masked vinculin binding site . Since force-bearing sites of wound edge cells contain VASP recruited by zyxin to promote actin polymerization , vinculin may also facilitate actin dynamics, possibly through its barbed-end nucleation activity , necessary for the force transmission.
At quiescent cell-cell contacts, jasplakinolide-induced actin stabilization promotes vinculin accumulation. The actin side-binding/bundling activity of vinculin may be important for the maintenance of stable actin network at cell-cell contacts. A low vinculin concentration at MDCK cell-cell contacts is consistent with unstable actin dynamics observed at mature cell-cell contacts . The physiological mechanism of actin stabilization remains unclear, but α-catenin dimers have been shown to interact with actin filaments to promote actin bundle formation . Therefore, both α-catenin and vinculin may work together to maintain the stable actin network at mature cell-cell junctions. Furthermore, in Drosophila embryos, two distinct actin dynamics are present to regulate cadherin mobility . These two distinct vinculin pools may be critical for the key divergence of actin function and may be a fundamental feature of actin regulation at cell-cell contacts.
The major findings of this study are that vinculin is required for both proper cell-cell adhesion and wound closure through contraction of the actomyosin purse-string. Additionally, vinculin recruitment to quiescent cell-cell contacts occurs with F-actin stabilization in MDCK cells. These results demonstrate vinculin to actively participate in generating force at cell-cell contacts and that vinculin localization at cell-cell contacts may occur in both a myosin II activity-dependent and independent manner.
Cell lines and reagents
MDCK GII cells were cultured in Dulbecco's modified Eagle's medium (low glucose) supplemented with 10% fetal bovine serum, penicillin, streptomycin, and kanamycin. MDCK cells stably expressing GFP-vinculin was previously described . Primary antibodies used were mouse monoclonal IgGs against vinculin (clone hVIN-1, Sigma), E-cadherin (clone 36, BD Biosciences), and tubulin (clone DM1A, Sigma). Myosin II activity was perturbed with 50 μM blebbistatin (Calbiochem) or 10 nM calyculin A (Calbiochem) while 200 nM jasplakinolide (Molecular Probes) was used to stabilize F-actin.
Canine vinculin-specific oligonucleotide sequences (shRNA1: 5'-TGAAGAGAGATATGCCACC-3', shRNA2: 5'-AAGGCAAGATTGAGCAAGC-3', or shRNA3: 5'-CAAGCTGCTTACGAACACT-3') were inserted into the pSUPER.gfp/neo expression vector (Oligoengine), and transfected into MDCK cells using Lipofectamine 2000 (Invitrogen). Stable vinculin knockdown cells were subcloned and screened for low vinculin levels using Western blot (see an example blot in Figure S1). The total number of subclones was 25 (shRNA1), 19 (shRNA2), and 16 (shRNA3) clones. Stable cell lines were maintained with 100 μg/ml G418 treatment.
Microscopy and live-cell imaging
Cells were imaged in a 37°C temperature-controlled chamber using a Zeiss AxioObserver equipped with a Yokogawa spinning disk confocal system, 40× and 63× objectives, 488 and 561 nm solid-state lasers, and a Cool SNAP HQ camera. The microscope system was controlled by Slidebook software (Intelligent Imaging Innovations).
A photo-ablation system (Intelligent Imaging Innovation) was used to ablate a single cell within a cell monolayer. The subsequent wound area was imaged every minute for 1 hr and the area of the wound opening was quantified using ImageJ. All images were acquired and generated using the same exposure, laser power, and gain. Wound size measurements within the first 10 min following cell ablation were used to analyze the initial velocity of wound closure.
Hanging drop assay
Cells were suspended at a density of 2.5 × 105 cells/ml medium. 25 μl of cell suspension was seeded onto glass-bottom dishes, inverted upside-down, and incubated at 37°C. Cell suspensions were then triturated through a pipette tip 30 times and the cluster sizes were quantified using ImageJ.
Immuno-fluorescence and image analysis
MDCK cells were seeded onto collagen-coated coverslips. Cells were fixed in 3% para-formaldehyde containing 0.3% TritonX-100 for 10 min and blocked with 1% BSA containing 0.3% TritonX-100. Following primary antibody incubation, immuno-labeled cells were detected with AlexaFluor 488-conjugated secondary antibodies (Molecular Probes). Cells were counter-stained with AlexaFluor 568-phalloidin (Molecular Probes) to detect F-actin. Fluorescence intensities were quantified using ImageJ. All images were acquired and generated using the same exposure and laser power. Fluorescence intensities were measured at cell-cell junctions by obtaining selection areas specific to F-actin staining at cell-cell contacts. The same selection areas obtained with F-actin images were overlaid onto corresponding vinculin images to obtain vinculin intensities along the cell-cell contacts (example images shown in Additional file 1, Figure S3).
Results comparing two groups were analyzed using a two-tailed, unpaired Student's t-test assuming equal variance. Results comparing three groups were analyzed using a one-way ANOVA and significance between groups was determined using the Bonferroni correction. Results were considered significant when P < 0.05. Data was analyzed using Microsoft Excel.
List of abbreviations
green fluorescent protein
Madin-Darby canine kidney
myosin light chain.
Acknowledgements and Funding
We thank Arisa Uemura and Mimi Zhu for helping Western blot analysis and cloning of shRNA plasmids. This work was supported by a Beckman Young Investigator Award, a Hellman Family New Faculty Award, a NIH EUREKA Award GM094798, the University of California Cancer Research Coordinating Committee.
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