Multiple domains in the Crumbs Homolog 2a (Crb2a) protein are required for regulating rod photoreceptor size
© Hsu and Jensen; licensee BioMed Central Ltd. 2010
Received: 14 October 2009
Accepted: 29 July 2010
Published: 29 July 2010
Vertebrate retinal photoreceptors are morphologically complex cells that have two apical regions, the inner segment and the outer segment. The outer segment is a modified cilium and is continuously regenerated throughout life. The molecular and cellular mechanisms that underlie vertebrate photoreceptor morphogenesis and the maintenance of the outer segment are largely unknown. The Crumbs (Crb) complex is a key regulator of apical membrane identity and size in epithelia and in Drosophila photoreceptors. Mutations in the human gene CRUMBS HOMOLOG 1 (CRB1) are associated with early and severe vision loss. Drosophila Crumbs and vertebrate Crb1 and Crumbs homolog 2 (Crb2) proteins are structurally similar, all are single pass transmembrane proteins with a large extracellular domain containing multiple laminin- and EGF-like repeats and a small intracellular domain containing a FERM-binding domain and a PDZ-binding domain. In order to begin to understand the role of the Crb family of proteins in vertebrate photoreceptors we generated stable transgenic zebrafish in which rod photoreceptors overexpress full-length Crb2a protein and several other Crb2a constructs engineered to lack specific domains.
We examined the localization of Crb2a constructs and their effects on rod morphology. We found that only the full-length Crb2a protein approximated the normal localization of Crb2a protein apical to adherens junctions in the photoreceptor inner segment. Several Crb2a construct proteins localized abnormally to the outer segment and one construct localized abnormally to the cell body. Overexpression of full-length Crb2a greatly increased inner segment size while expression of several other constructs increased outer segment size.
Our observations suggest that particular domains in Crb2a regulate its localization and thus may regulate its regionalized function. Our results also suggest that the PDZ-binding domain in Crb2a might bring a protein(s) into the Crb complex that alters the function of the FERM-binding domain.
Vertebrate photoreceptors are morphologically complex and highly compartmentalized cells with large apical domains. The apical domain consists of a proximal inner segment and a distal outer segment, which is a modified cilium packed with intramembranous discs containing the photon-capturing opsins. Photoreceptors are not renewable like many other cell types, such as skin and intestinal cells, but each individual photoreceptor has the remarkable, and perhaps unique, ability to shed and renew a part of themselves- the outer segment. Photoreceptors shed the tips of their outer segments, which are then phagocytosed by the neighboring retinal pigmented epithelium [1, 2]. It has been estimated that 10% of the rod outer segment is shed each day . Remarkably little is known about the molecular and cellular mechanisms that control vertebrate photoreceptor morphogenesis and even less about how photoreceptors control the size of their outer segments through the processes of shedding and renewal.
Crumbs proteins are regulators of apical identity and size [4–6]. Previously we showed that the FERM protein Mosaic eyes (Moe; known as Epb4.1L5 in mammals and Yurt in Drosophila) is a novel component of the Crumbs complex and that loss-of-moe function results in an expanded apical domain, the outer segment, in rods [7, 8]. Our observations led us to suggest that Crumbs may be a part of the outer segment renewal machinery in photoreceptors. Mutations in one of the three human crumbs-like genes, CRB1, cause severe and early onset vision loss diseases [9–12]. Missense mutations in CRB1 associated with disease are found in all domains, suggesting that all domains, including the extracellular and intracellular domains, contribute to CRB1 function (for review see ). Drosophila Crumbs is required for normal photoreceptor morphogenesis and the role of particular domains in Crumbs in photoreceptor morphogenesis has been examined [5, 14, 15]. The Crumbs homologs expressed in vertebrate photoreceptors share structural homology with Drosophila Crumbs [6, 10, 16]. The role of particular domains in vertebrate Crumbs homolog 2 (Crb2) proteins in regulating vertebrate photoreceptor morphogenesis has not been examined. To determine the function of specific domains in Crb2 proteins in vertebrate photoreceptors we generated transgenic zebrafish lines in which rods overexpress particular domains of the Crb2a protein. Our analyses suggest that multiple domains in Crb2a are important for protein localization and for its function in regulating the size of apical cellular compartments.
Localization of extracellular crb2a transgene products in rods
The role of the extracellular domain in vertebrate Crb1 and Crb2 proteins is unknown and no molecules have been identified that interact with this region. In Drosophila, the extracellular domain plays a critical role in determining the length of the stalk region in photoreceptors . The structure of zebrafish Crb2a protein is similar to Drosophila Crumbs and has a large extracellular domain with 19 EGF-like domains and 3 Laminin-like domains, a single pass transmembrane domain, and a small intracellular domain with a FERM binding domain (FBD) and a PDZ-binding domain (PBD; Fig. 1A). We asked whether the role of Crb2a extracellular domain is conserved across species and sought to determine the role of the extracellular domain of Crb2a in protein localization and rod morphogenesis. We generated transgenics expressing the full-length Crb2a protein (Crb2aFL), the extracellular domain of Crb2a containing the transmembrane domain (Crb2aExtra_TM), and the extracellular domain of Crb2a that lacks the transmembrane domain and, thus, is a secreted Crb2a extracellular domain (Crb2aExtra_Secr; Fig. 1B),
The rod photoreceptor has four morphologically and functionally distinct compartments. There are two basal compartments, the proximal cell body and the distal synaptic region, and two apical compartments, the proximal inner segment and the distal outer segment that is filled with membranous discs packed with photon-capturing Rhodopsin molecules (Fig. 1C). The apical and basal compartments are separated by a specialized adherens junction called the outer limiting membrane (OLM). The inner segment can be further subdivided into the proximal myoid region and the distal ellipsoid region (Fig. 1C). All the transgenic lines were made in or crossed into the Tg(Xop:EGFP) line  for analysis of rod morphology. The four compartments are visible in rods in the Tg(Xop:EGFP) background when labeled with anti-Rhodopsin antibodies , the OLM is also visible as a small gap or constriction in GFP fluorescence (Fig. 1D, arrow).
At 6 d, which is about 3 days after the first rod birthdates, Crb2aFL protein localized primarily to the inner segment, intensively in the proximal myoid and at a lower level in the ellipsoid, and very little was found on the plasma membrane of the cell body and none was found in the outer segment (Fig. 1E-E"'). The myoid region of the inner segment of Crb2aFL expressing rods was enlarged (Fig. 1E-E"'). This morphology was so unique and distinct that we could easily distinguish Crb2aFL rods from wild-type or other transgenic lines by observing GFP fluorescence alone. We also observed that ectopic processes often projected from the inner segments of Crb2aFL expressing rods (Fig. 1E"').
Crb2aExtra_TM was very strong in the outer segment where we observed very fine stripes of Crb2aExtra_TM that appear more concentrated on one side of disks in the outer segment, perhaps near the axoneme, and there was weak plasma membrane labeling in the cell body and inner segment (Fig. 1F-F" and Additional file 1B, B'). Crb2aExtra_Secr localized throughout the entire expressing rod except the outer segment (Fig. 1G-G"). We observed Crb2aExtra_Secr in the region where Crumbs (predominantly Crb2a) proteins normally localize, just apical to the OLM (Fig. 1G", white bracket) and surrounding the inner segments of cones (Fig 1G", yellow bracket and Additional file 2). We observed no Crb2aExtra_Secr below the OLM except within the cell bodies of the expressing rods. Single z-sections of Crb2aFL-, Crb2aExtra_TM- and Crb2aExtra_Secr-expressing rods are shown in Additional file 1A-C'.
Localization of intracellular crb2a transgene proteins in rods
In order to investigate the role of the intracellular domain, consisting of the FERM-binding and PDZ-binding domains, we over-expressed several Crb2a constructs that lack the extracellular domain (Fig. 2A). We found that the localization of the Crb2a constructs that lack the extracellular domain is very different from those that retain the extracellular domain. At 6 d, Crb2aIntraWT localized to the cell body plasma membrane, the proximal inner segment including the Golgi apparatus (see Additional file 3 for Golgi labeling in Crb2a transgenics), and outer segment (Fig. 2C-C"). Stripes of Crb2aIntraWT were often observed in the outer segments (Fig. 2C') and the number of stripes correlated with the number of light cycles to which the cells had been exposed. The intracellular construct that lacks a functional FERM binding domain, Crb2aIntraΔFBD, localized similarly to Crb2aIntraWT, with labeling in the cell body plasma membrane, proximal inner segment (presumptive Golgi apparatus), and outer segment (Fig. 2D-D"). The amount of Crb2aIntraΔFBD protein in the cell body plasma membrane appeared lower than Crb2aIntraWT. We also observed stripe patterns of Crb2aIntraΔFBD in the outer segments (Fig. 2D'), suggesting that this construct may be regulated similarly to Crb2aIntraWT. The intracellular construct that lacks the PDZ binding domain, Crb2aIntraΔPBD, localized mostly to the plasma membrane of cell body and the inner segment and there was very little labeling in the outer segment (Fig. 2E-E"). We also made a control construct that lacks a functional FERM binding domain and PDZ binding domain, Crb2aIntraΔFBDΔPBD, and found this construct localized to the plasma membrane of the cell body, proximal inner segment (presumptive Golgi apparatus), and outer segment (Fig. 2F-F"), similar to Crb2aIntraWT and Crb2aIntraΔFBD. Single z-sections of Crb2aIntraWT-, Crb2aIntraΔFBD-, Crb2aIntraΔPBD- and Crb2aIntraΔFBDΔPBD-expressing rods are shown in Additional file 1D-G'. We note that the levels of Crb2aIntraWT, Crb2aIntraΔFBD and Crb2aIntraΔFBDΔFBD in the cell bodies are much lower than Crb2aIntraΔPBD, which may not be clear from the confocal images (Fig. 2). The only intracellular construct that did not localize to the outer segment was Crb2aIntraΔPBD, suggesting that this construct may be actively retained in the inner segment and cell body by the remaining FBD or that this construct is trafficked differently than the other constructs.
Mutations in the FERM binding domain of Crb2a (Crb2aIntraΔFBD) abolish in vitro binding to the FERM protein Moe
Localization of Rhodopsin is unaffected in transgenic rods
Since many of our Crb2a transgene products are mislocalized to the outer segment and cell body, we asked whether over-expression of any of the Crb2a constructs interferes with Rhodopsin localization in the outer segment. To recognize Rhodopsin we used a Rhodopsin-specific monoclonal antibody (clone R6-5 ). In wild-type rods at 6 d, Rhodopsin localized only to the outer segment (Fig. 1B, 2B), and we found that Rhodopsin localization is normal in all our transgenic lines (Figs. 1 and 2 and data not shown), indicating that over-expression of either the intracellular or extracellular domains of Crb2a does not interfere with Rhodopsin transport or targeting even though many transgene products localized to the outer segment.
Overexpression of the Crb2a intracellular domain increases outer segment size
Overexpression of the Crb2a extracellular domain increases the size of inner and outer segments
We measured the size of Crb2aFL, Crb2aExtra_TM and Crb2aExtra_Secr expressing rods at 6 d. The outer segments of Crb2aFL rods were not significantly larger than wild-type rods but outer segments of Crb2aExtra_TM and Crb2aExtra_Secr rods are larger than those in wildtypes (Fig. 4B). The most dramatic morphological change we observed was in Crb2aFL rods, where the myoid region of the inner segment was enlarged (Fig. 1E-E"). The enlargement of the myoid in Crb2aFL rods was most obvious in the area immediately above the OLM, therefore we measured the width the myoid just apical to the OLM in confocal z-projections as indicated in Fig. 4C. The width of the myoid in Crb2aFL expressing rods was nearly 50% wider than wildtypes (Fig. 4C, C'). The widths of the myoid in Crb2aExtra_TM or Crb2aExtra_Secr rods were not significantly different from wildtypes (Fig. 4C, C'). We also quantified the size of the inner segment by measuring the area of the entire myoid in confocal z-projections as indicated in Fig. 4D. This measurement showed that Crb2aFL, Crb2aExtra_TM, Crb2aExtra_Secr rods had a significant increase in the size of myoid (Fig. 4D').
Monoclonal antibody zs-4 recognizes the extracellular domain of Crb2a
Our next goal was to determine whether expression of any of our transgenes affected the levels or localization of endogenously expressed Crb2a/b proteins. The panCrb antibodies that we used previously and the one we raised and used in this study were generated against a C' terminal peptide that includes the PBD that is highly conserved in all members of the Crumbs family of proteins and these antibodies recognize all zebrafish Crumbs proteins  (and data not shown). Thus, the panCrb antibody recognizes all constructs that retain the C' terminal peptide and cannot be used to distinguish between those constructs and endogenous Crumbs proteins. In order to distinguish between endogenous Crb2a/b proteins and intracellular transgene products, we required an antibody that recognizes the extracellular domain of Crb2a.
Expression of Crb2aFL may alter the localization of endogenous Crumbs proteins
Overexpression of intracellular Crb2a constructs does not alter localization of endogenous Crb2a proteins, Prkci or Moe
We asked whether overexpression of Crb2a constructs alters the localization of other Crumbs complex components such as Moe or those that interact indirectly, such as Prkci (also known as aPKCΔλ) [22–24]. We used antibodies against Moe and Prkci to localize these two proteins in our transgenics. We found that the localization of Moe and Prkci was unaffected in all transgenic lines (Additional file 4 and Additional file 5, respectively).
In this study we sought to determine which domains in Crb2a contribute to its localization and function in rods. Zebrafish rods express two crb2 paralogs, crb2a and crb2b, whereas crb1 expression was not detected in the retina [6, 8]. Mouse rods also express two crumbs orthologs, crb1 and crb2[8, 25, 26]. The observation that in zebrafish cells in the retina express two crb2 genes but no crb1 suggests that one or both of the crb2 genes has adopted the function of crb1. While mutations in human CRB1 are associated with several severe and early onset retinal degeneration diseases [9, 10] (and reviewed in ), as yet no human disease has been associated with CRB2 mutations. Loss-of-crb1 function in mice also causes retinal defects but they appear less severe than those observed in humans; the OLM is disrupted in the rd8 mouse and inner and outer segments are smaller than normal [28, 29]. We observed no defects in the OLM in any of the crb2a transgenic lines we created; anti-ZO-1 labeling was normal (data not shown) and adherens junctions in transgenic rods were visible as a distinct gap in GFP in the inner segment. As yet, loss-of-crb2 function in mice has not been reported, so its role in mammalian photoreceptors remains unknown.
Loss-of-crb2b function resulted in reduced photoreceptor apical size and we previously reported that loss-of-moe function, a putative negative regulator or Crumbs protein function, resulted in larger than normal outer segments [6, 8, 30]. In this study, overexpression of Crb2aIntraWT, Crb2aIntraΔFBD, Crb2aIntraΔPBD, Crb2aExtra_TM, or Crb2aExtra_Secr resulted in a significant increase in outer segment size and without interfering with normal development of rods. This result supports our hypothesis that Crb2a may be involved in the renewal mechanism in photoreceptors. Overexpression of Crb2aIntraWT, Crb2aIntraΔFBD, Crb2aIntraΔPBD proteins may increase outer segment size by competing for negative regulators of endogenous Crumbs proteins. For example, the FBD in Crb2aIntraΔPBD may compete with endogenous Crb2a for binding to Moe, a suggested negative regulator of Crumbs protein function [6, 8, 30], and, thus, could lead to potentially more activity of endogenous Crb2a and a larger outer segment. It is more difficult to envision a mechanism by which overexpression of Crb2aExtra_TM and Crb2aExtra_Secr increases outer segment size without knowing what molecules interact with the extracellular domain of Crumbs proteins. Transgene expression likely increases outer segment size by increasing outer segment growth rather than decreasing outer segment shedding because at 6 d shedding has yet to begin; we see no RPE phagosomes at 6 d by immunocytochemistry or TEM (AMJ, unpublished observation).
Crb family proteins and several components of the Crumbs complex have a restricted localization just apical to adherens junctions in epithelia or the outer limiting membrane (OLM) in photoreceptors [6, 8, 28, 29, 31–33]. Crb family proteins are found in photoreceptor inner segments immediately apical to the OLM and in Müller glial microvillar processes that project into the inner segment region [8, 32]. In zebrafish rods there is a morphologically distinct region just apical to the OLM that can be recognized as a bulge in the proximal inner segment (see Fig. 1D, arrow). In mouse, Crb2 localized by immunoEM to this region . Our current findings suggest that domains in both the extracellular region and intracellular regions of Crb2a contribute to its proper localization in rods. Only Crb2aFL localization in the myoid region approximated the normal localization of endogenous Crb2a/b proteins, although its localization was expanded and this expansion caused the bulge to expand. The functional significance of this region in photoreceptors is unknown.
Crb2aIntraΔPBD was retained in the inner segment and cell body plasma membrane and very little was found in the outer segment in contrast to Crb2aIntraΔFBD, which localized mostly to the outer segment. These results suggest that the FBD is responsible for retaining Crb2a in the inner segment. Crb2aIntraWT is also found in the outer segment, however, and it retains the FBD (and the PBD). Why? One possible explanation is that proteins brought into the Crb complex by the PBD disrupt or alter the interaction between the FBD and its binding partner and thus Crb2aIntraWT behaves more like Crb2aIntraΔFBD. The PBD could bring PRKCi into the Crb complex, PRKCi could phosphorylate the FBD and thus lower its affinity for Moe, which localizes cortically in the inner segment and cell body , and, thus, Crb2aIntraWT localizes similarly to Crb2aIntraΔFBD and Crb2aIntraΔFBDΔPBD. Drosophila Crumbs has been shown to be a substrate for phosphorylation by aPKC (orthologue of PRKCi) and Crb activity during embryogenesis is regulated by phosphorylation . Finally, the observation that several transgene products localize to the outer segment in the absence of any outer segment 'targeting signal' suggests that the outer segment could be the default localization for proteins that lack cytoskeletal (or extracellular) anchorage. Observations by Baker and colleagues lead them to also suggest that the rod outer segment seems to be the default localization for single-pass transmembrane proteins .
Crb2aExtra_TM also localizes to the outer segment and this finding is different from that observed in fly photoreceptors where an equivalent construct localized to the stalk membrane . We also found that Crb2aExtra_TM expression in the outer segment is very different from Crb2aIntraWT and Crb2aIntraΔFBD; Crb2aExtra_TM protein forms fine stripes that are concentrated on one side of the outer segment, perhaps near the axoneme. We have no explanation of why Crb2aExtra_TM is more concentrated on one side of the outer segment other then suggesting that since Crb2aExtra_TM is a much larger protein than Crb2aIntraWT, Crb2aIntraΔFBD and Crb2aIntraΔFBDΔFBD and, consequently, it would be less likely to diffuse freely in the disk membrane.
The localization of Crb2aExtra_Secr is intriguing. It is found in the cell body and in and around the inner segment of Crb2aExtra_Secr rods as well as around the inner segments of neighboring cones. It is possible that the secreted Crb2a extracellular domain is trapped by an unknown receptor located on cone inner segments or Müller processes in the region. Functionally, overexpression of Crb2aExtra_Secr in rods led to a small increase in the size of the outer segment and a modest increase in the area of the myoid region, which is opposite to the effect observed in Drosophila where overexpression of this construct shortened the stalk . One possible explanation could be that most Crb2aExtra_Secr protein is sequestered away from the site where it could interfere with normal Crb2a signaling at the base of the myoid in rods; most Crb2aExtra_Secr protein localizes to the region near rod ellipsoids and cone inner segments.
While we cannot exclude entirely the possibility that differences in protein folding between the different constructs contribute to differences in localization, we think it is unlikely for the following reasons. First, given that the extracellular domain is identical in the three constructs that retain that domain (Crb2aFL, Crb2aExtra_TM, and Crb2aExtra_Secr) it seems unlikely that these proteins would fold differently in the ER. It is also unlikely that the presence of the intracellular domain would alter folding kinetics in the ER but we cannot exclude the possibility that it could affect time spent in the ER. Two, it seems unlikely that the small intracellular domain (37 amino acids at the longest) is subject to folding, given what is known about other FERM-binding domains and PDZ-binding domains. Third, the zs-4 antibody, which only recognizes the native (i.e. folded) extracellular domain of Crb2a, recognizes all constructs that retain the extracellular domain, suggesting that the extracellular domain is folded properly.
Overexpression of Crb2aFL had the greatest effect on rod morphology and dramatically increased the width and area of the inner segment myoid region. Similarly, overexpression of full-length Crb had the greatest effect on stalk length in Drosophila photoreceptors . It has been suggested that the inner segment of vertebrate photoreceptors may be a homologous structure to the stalk region in ommatidial photoreceptors in insects, as both lie in between the sensory compartment (outer segment in vertebrates and rhabdomere in insects) and the cell body . The mechanism of inner segment enlargement in Crb2aFL-expressing rods remains unclear. It seems unlikely that it is due to enlargement of the endoplasmic reticulum as two other constructs (Crb2aExtra_TM and Crb2aExtra_Secr) that are identical in the extracellular domain and expressed at similar levels do not produce such an enlargement (Additional file 6). It also seems that it is unlikely to be due to enlargement of the Golgi apparatus for similar reasons and we did not observe an enlarged Golgi apparatus in Crb2aFLexpressing rods (Additional file 3). We also observed that overexpression of Crb2aFL resulted in the appearance of fine processes emerging from the inner segment myoid. Interestingly, we also observed ectopic processes in the inner segment myoids of rods that lack Moe function, the FERM protein shown to bind the FBD of Crumbs proteins, and which was suggested to act as a negative regulator of Crumbs proteins [8, 36]. The molecular origin of these processes remains unclear.
The mechanism by which Crumbs proteins regulate apical cell polarity and apical membrane size remains mysterious. In Drosophila photoreceptors, both the extracellular and intracellular domains are important for Drosophila photoreceptor development and morphogenesis, in contrast, the extracellular domain seems largely dispensable for embryogenesis [4, 5, 15, 37]. Our observations suggest that the functions of particular domains in Crb2a in regulating photoreceptor morphology are partly conserved with those in Drosophila Crb. Our results show that multiple domains in Crb2a are required for its location and function in rods. Since the extracellular domain of Crb2a is important for function, as in Drosophila photoreceptors , the identification of interactors that bind to the extracellular domain is especially important.
AB wild-type strain, Tg(Xop:EGFP);alb-/+, the Tg(Xop:Crb2a), ome m98 fish lines were maintained and staged as previously described according to Westerfield . All experiments involving animals were performed with approval by and in accordance with the University of Massachusetts-Amherst Institutional Animal Care and Use Committee (IACUC).
Diagrams of the transgene constructs are shown in Figs. 1B and 2A. The constructs were cloned behind 0.8 kB of the Xenopus rod opsin promoter (Xop; ). All constructs have an N'terminal signal peptide (SP) taken from zebrafish Crb2b (SignalP 3.0 Server) and an influenza hemagglutinin (HA) tag (YPYDVPDYA) just after the SP. To make Crb2aFL, the SP of Crb2a was substituted with the Crb2b SP and the HA tag was engineered immediately after the predicted SP cleavage site. To make Crb2aExtra_Secr and Crb2aExtra_TM, we used site-directed mutagenesis to place a stop codon just before and after the transmembrane domain, respectively. To make Crb2aIntraWT, the SP from zebrafish Crb2b was introduced with PCR using a pair of primers in which the HA tag sequence was engineered immediately after SP within the reverse primer and the sequence containing the Crb2a transmembrane domain plus the intracellular domain was amplified by PCR and cloned in-framed following the HA sequence. Crb2aIntraΔPBD was made using Crb2aIntraWT as a template and site-directed mutagenesis was performed to introduce a stop codon after the last glutamic acid residue (E16) in the FERM-binding domain. To make Crb2aIntraΔFBD, the conserved amino acids tyrosine (T10) and glutamic acid (E16) of Crb2aIntraWT were substituted with alanines to compromise the FERM-binding domain as described by Izaddoost and colleagues . Site-directed mutagenesis was performed on Crb2aIntraΔPBD to introduce a stop codon after E16 to make Crb2aIntraΔFBDΔPBD. All constructs contain a poly-adenylation sequence at the 3'-end. The constructs were cloned into pTol [18, 19].
Transgenic zebrafish lines were generated using pTol system [18, 19]. We coinjected 40 ng/mL of pTol-transgene construct plasmid with 40 ng/mL transposase mRNA into one-cell stage Tg(Xop:EGFP);alb-/+ embryos. Injected embryos were grown to adulthood and out-crossed with Tg(Xop:EGFP);alb-/+ to produce offspring. We used PCR to identify transgenic offspring; forward primer GGCATGCCGTCCCTAAAAG designed within the promoter region, and the reverse primer AGCGTAATCTGGAACATCGTAT within the HA tag sequence. We identified three germline transgenic founders (F0) for each construct and generated F1 lines. We confirmed transgene expression by anti-HA immunohistochemistry. Transgenic F1s and subsequent generations were identified by PCR on fin clip DNA. F1 carriers were out-crossed with Tg(Xop:EGFP);alb-/+ line to produce F2s.
In Vitro GST Interaction
Construction and expression of Crb2a fusion proteins and HIS-Moe_FERM were described previously . GST-Crb2aIntraΔFBD was made using site-directed mutagenesis on GST-Crb2aIntraWT (as described above) to introduce mutations in two residues of FBD (E10 and T16). Protein interactions were performed as described previously , using 10 mg of His-Moe_FERM incubated with 10 mg of GST, GST- Crb2aIntraWT or GST-Crb2aIntraΔFBD.
Immunocytochemistry and Microscopy
Production and levels of transgene products were assessed on 6 d retinal sections by anti-HA antibody labeling. We fixed 6 d zebrafish in the afternoon in 4% paraformaldehyde. Cryostat sections (20-30 μm) were treated with 0.1% SDS for 15 min, washed in PBS with 0.1% Tween (PBS-Tw), incubated in 10% goat serum in PBS-Tw, rinsed briefly in PBS-Tw, and incubated overnight at 4°C in primary antibody (monoclonal anti-HA IgG1, 1:1000 (Covance); monoclonal anti-HA IgG3, 1:500 (Upstate); rabbit anti-Moe, 1:1000; rabbit anti-panCrb (we raised against the synthetic peptide, AGARLEMDSVLKVPPEERLI), 1:500; anti-aPKCζ, 1:1000 (Santa Cruz); rabbit anti-GFP 1:200 (Molecular Probes), anti-Rhodopsin monoclonal R6-5, 1:50 ; zs-4 antibody, 1:10 (University of Oregon Monoclonal Antibody Facility); rabbit anti-GOLGA5 1:500 (Sigma HPA000992). Sections were washed, incubated with the appropriate secondary antibodies (-FITC/-TRITC (Molecular Probes) 1:100, -CY5 1:100 (Jackson) goat anti-mouse IgG3 rhodamine red-conjugated, 1:100; goat anti-mouse IgG1 Cy-5-conjugated, 1:250; goat anti-mouse IgG1 rhodamine red-conjugated, 1:100; goat anti-mouse IgG2a Cy-5-conjugated, 1:100 (Jackson Laboratory)), and analyzed with a Zeiss LSM 510 Meta Confocal System. We primary analyzed the retinas in alb-/- individuals to ensure that the entire outer segment was visible and not obscured by the RPE; the localization of transgene products was the same in pigmented siblings (data not shown). Confocal images are a single scan (averaged 4 times) at about 1 μm optical thickness. Volume of wild-type and transgenic rods was measured using Sync Measure 3D function of Image J. Only cells that were completely captured in the confocal stacks were measured. Outer segments were outlined and measured by an overlap of anti-GFP and R6-5 labeling; cell compartments that are free of R6-5 labeling were outlined and measured as inner segment plus cell body. The width of inner segment (Fig. 4C) and the area of myoid regions (Fig. 4D) were outlined and measured using Image J.
Outer limiting membrane
Extracellular domain secreted
Extracellular domain transmembrane domain
We thank the following: Paul Hargrave for anti-Rhodopsin monoclonals; Jim Fadool for Xop:EGFP transgenic fish; David Papermaster for the Xenopus rod opsin promoter; Judy Bennett for fish care; Arne Christensen for the rod illustration in Fig. 1. Supported by the NIH, EY015420.
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