Peptide aptamers as new tools to modulate clathrin-mediated internalisation — inhibition of MT1-MMP internalisation
© Wickramasinghe et al; licensee BioMed Central Ltd. 2010
Received: 27 October 2009
Accepted: 23 July 2010
Published: 23 July 2010
Peptide aptamers are combinatorial protein reagents that bind to targets with a high specificity and a strong affinity thus providing a molecular tool kit for modulating the function of their targets in vivo.
Here we report the isolation of a peptide aptamer named swiggle that interacts with the very short (21 amino acid long) intracellular domain of membrane type 1-metalloproteinase (MT1-MMP), a key cell surface protease involved in numerous and crucial physiological and pathological cellular events. Expression of swiggle in mammalian cells was found to increase the cell surface expression of MT1-MMP by impairing its internalisation. Swiggle interacts with the LLY573 internalisation motif of MT1-MMP intracellular domain, thus disrupting the interaction with the μ2 subunit of the AP-2 internalisation complex required for endocytosis of the protease. Interestingly, swiggle-mediated inhibition of MT1-MMP clathrin-mediated internalisation was also found to promote MT1-MMP-mediated cell migration.
Taken together, our results provide further evidence that peptide aptamers can be used to dissect molecular events mediated by individual protein domains, in contrast to the pleiotropic effects of RNA interference techniques.
Peptide aptamers (PAs) are small, artificially engineered proteins conceptually similar to antibodies . PAs consist of a stable, ideally inert scaffold protein with an inserted constrained peptide moiety. This in effect presents a small peptide surface within the tertiary structure of the scaffold which serves as the binding site for a target protein. In contrast to most of the more than 40 non-antibody scaffolds described to date , PAs are usually isolated by yeast-two hybrid screening of large libraries of PAs that contain random peptide inserts against a bait protein of interest. Selection of PAs in eukaryotic cells in vivo may allow the identification of interactors that are more easily transferable to mammalian cells than interactors identified using in vitro techniques such as phage-display. PA technology is well established, with PAs showing biological activity against a wide variety of proteins from different organisms, including the human and D. melanogaster Cdk2 proteins [1, 3], the E. coli thymidylate synthase (ThyA) protein , the E6 and E7 proteins from human papilloma virus (HPV) [5, 6], the human EGF receptor , and the transcription factors Stat3  and the BCL-6 . Importantly, some PAs have also been found to block functions of their target proteins in vivo, such as human Cdk2 , D. melanogaster Cdk1 and 2 , E2F , p53 , Stat3 , Nr-13 , and BCL-6 .
Membrane-type 1 Matrix Metalloproteinase (MT1-MMP, also known as MMP-14), is a member of the large MMP family of enzymes. MT1-MMP plays a major role in the dynamic remodelling of the extra-cellular matrix (ECM) and has been reported to directly degrade a broad spectrum of ECM proteins, including collagen types I, II, and III, fibronectin, laminin 1, laminin 5, fibrin, and aggrecan [14, 15]. MT1-MMP has also been reported to activate proMMP-2 and proMMP-13 [16, 17], thereby indirectly increasing its proteolytic repertoire on or near the cell surface. The protease also plays a role in the processing of a growing number of membrane proteins, including, for example, CD44 , transglutaminase , the integrin αV chain  or syndecan 1  thus modulating cell signalling and the cellular functions mediated by these molecules.
MT1-MMP has been implicated in a wide spectrum of physiological and pathological cellular functions [22, 23]. MT1-MMP expression, well documented in many tumours, has been correlated with key in vitro and in vivo processes of tumour progression including angiogenesis , cell migration and invasion , cell growth  and metastatic spread [27, 28]. Inhibition or silencing of the protease has been found to significantly reduce the invasive phenotype of tumour cells implicating a leading role for MT1-MMP in such processes [25, 29].
MT1-MMP is a type I transmembrane protein with a very short intracellular domain (ICD) of just 21 amino acids. The MT1-MMP ICD has been reported to be required for cell migration and invasion [30–33] as well as tumour growth . The identification of proteins interacting with the MT1-MMP ICD, such as MTCBP-1 , and glCqR  have also helped in defining new localisations and cellular functions for this protease. The MT1-MMP ICD has also been implicated in the internalisation  and the recycling of the protease to the cell surface . Consistent with this, MT1-MMP ICD has been reported to interact with the μ2 subunit of the AP-2 complex  as well as with caveolin-1 
To date, crucial information on the cellular function of the intracellular domain of the protease has been obtained following exogenous expression of mutant MT1-MMP ICD constructs [31, 32, 39, 38, 37, 40, 41, 33] or constructs with a partially or completely deleted ICD [30, 42, 26, 43, 40, 34]. In order to assess the role of the MT1-MMP ICD without using exogenously truncated or mutated forms of the protease, we decided to make use of PA technology.
In this study, we identify and characterize a PA, named swiggle, which interacts with the 21 amino acid ICD of MT1-MMP. Expression of swiggle in human cells was found to stimulate MT1-MMP mediated cell migration. Detailed analysis of the phenotypic effect of swiggle revealed that the PA inhibits internalization of MT1-MMP resulting in the accumulation of the protease at the cell surface. Our data indicate that swiggle interacts with the LLY573 motif in the MT1-MMP ICD and competes with the μ2 subunit of the AP-2 complex in cells, thereby inhibiting the endocytosis of MT1-MMP.
Isolation of peptide aptamers that interact with the MT1-MMP ICD
To confirm the interaction of the selected PAs with MT1-MMP ICD, yeast interaction mating experiments were performed . Haploid yeast strains expressing the LexA DNA-binding domain alone (LexA-DBD), LexA DBD fusions to the MT1-MMP ICD (LexA-MT1) or Cdk4 (LexA-Cdk4) were mated with strains expressing B42 activation domain (AD) fusions to TrxA (AD-TrxA), swiggle (AD-swiggle), 76 (AD-76) or Cyclin D1 (AD-CyclinD1). We included in this assay a fusion of the AD to a mutant variant of swiggle, called s14, identified by random mutagenesis, which no longer binds to the LexA-MT1 fusion protein. s14 differs from swiggle by a single amino acid: GGLIPCYFMH in swiggle to GGLIPCYFTH in s14 (Figure 1A). Resulting diploids were grown on selective media to identify positive (Figure 1B, grey squares) or negative (Figure 1B, white squares) interactions. As expected, a clear interaction was observed between the LexA-Cdk4 and AD-CyclinD1 pair used as a positive control (Figure 1B). LexA-MT1 did not bind to AD-TrxA, AD-s14 or AD-CyclinD1 but a clear interaction with AD-swiggle or AD-76 (Figure 1B) could be observed confirming that both PAs interact, via the inserted peptide, with the MT1-MMP ICD. In some experiments, a weak interaction between the LexA-DBD and AD-76 could also be observed, suggesting that PA 76 may also recognize sequences or surfaces from the LexA-DBD (data not shown). No interaction was detected between the LexA-DBD and AD-TrxA, AD-swiggle, AD-s14, AD-76 or AD-CyclinD1 (Figure 1B). AD-swiggle was also found to interact with the ICD of MT2-MMP and MT3-MMP (LexA-MT2 and -MT3 in Figure 1B). AD-76 clearly interacted with LexA-MT3 and a weak interaction with LexA-MT2 was observed. AD-swiggle and AD-76 did not interact with the ICD of MT5-MMP (LexA-MT5 in Figure 1B). Taken together, our data clearly demonstrate an interaction between the MT1-MMP ICD and both PAs. The weak interaction between AD-76 and LexA-DBD, coupled with the truncated structure of PA 76 and its instability when expressed in E. coli and mammalian cells (data not shown) led us to focus on swiggle in subsequent experiments.
GFP-swiggle co-immunoprecipitates with MT1-MMP in MCF7 cells
GFP-swiggle increases MT1-MMP-mediated cell migration
GFP-swiggle increases expression of MT1-MMP at the cell surface
Swiggle inhibits MT1-MMP internalization
Swiggle interacts with the MT1-MMP LLY573 internalization motif
The internalisation of MT1-MMP has been shown to be able to occur via clathrin- or caveolin-mediated pathways [49, 53, 31, 32]. Because MCF7 cells do not express detectable levels of caveolin-1 [54–58], we asked whether GFP-swiggle was able to interfere with the clathrin-mediated internalisation of the protease.
If swiggle and μ2 both bind to the LLY573 motif of MT1-MMP, then expression of swiggle should competitively inhibit the interaction between μ2 and MT1-MMP in cells. To test this idea, MCF7 cells were transiently co-transfected with MT1-MMP alone or with MT1-MMP together with GFP-s14 or GFP-swiggle. Total cell lysates were subjected to immunoprecipitation with the anti-MT1-MMP antibody and immune complexes were probed with anti-μ2, -MT1-MMP and -GFP antibodies. As shown in Figure 8B, we observed a clear co-immunoprecipitation of MT1-MMP and μ2 when the protease is expressed alone (Figure 8B, lane 2), or in the presence of GFP-s14 (Figure 8B, lane 3). In contrast, we were unable to detect μ2 in complexes immunoprecipitated from MCF7 cells expressing MT1-MMP and GFP-swiggle (Figure 8B, lane 4), thus demonstrating that expression of the PA inhibits the formation of a complex between the protease and the μ2 subunit. Taken together, our data suggest that the inhibition of MT1-MMP endocytosis observed following expression of swiggle probably results from the interaction of the PA with the LLY573 motif of the MT1-MMP ICD, thus disrupting the interaction of the MT1-MMP ICD with the μ2 subunit. Our results thus suggest a potential mechanism for the amplification of the effects of MT1-MMP on cell migration by GFP-swiggle. They also demonstrate that swiggle binding to the ICD can inhibit endocytosis of MT1-MMP without affecting communication between the ICD and the machinery required for cell migration.
In this study, we have described the isolation and characterisation of a PA, called swiggle, which binds to the ICD of the membrane-bound matrix metalloproteinase, MT1-MMP. Our results have several implications for PA technology. Firstly, we have demonstrated that PAs can be successfully obtained against small intracellular domains of transmembrane proteins. Although short peptides have previously been used in yeast two hybrid interaction assays, for example to map interaction domains, the 21 amino acid MT1-MMP ICD bait is approximately three times smaller than the previous smallest published bait used in a screen, a 59 amino acid C-terminal portion of the HPV-16 E7 protein . Our bait is also more than six times smaller than the only other published ICD used in a PA screen, the 133 amino acid ICD of the EGF receptor . Given the dearth of tools to directly study the biology of the MT1-MMP ICD, which could be attributed to its small size, this study raises the possibility that PA technology could be used to generate tools to study the biology of other small intracellular domains, such as integrins, that were previously difficult to study.
PAs have traditionally been found to produce inhibitory phenotypes by blocking certain functions of their target proteins [10, 3, 11, 5, 8, 13, 9]. For example, PAs that interacted with the human papilloma virus (HPV) E6 oncoprotein could block E6-mediated degradation of p53, resulting in increased p53 protein levels, growth inhibition and apoptosis in HPV-positive HPV16 cells . In contrast, we found that expression of GFP-swiggle resulted in an increase in both MT1-MMP-mediated TR-gelatin degradation and cell migration. Furthermore, GFP-swiggle appeared to affect the amount of MT1-MMP at the cell surface. This increased gain of function migratory phenotype caused by GFP-swiggle is unique, although it is consistent with the original prediction for the mechanism of PA function  in that it appears to result from a loss of function, namely reduction of the rate of endocytosis of MT1-MMP. Although a previous study has described a PA that stimulates its target protein, calcineurin, this PA was identified from an anti-proliferative phenotypic screen, rather than a yeast-two hybrid screen with a defined bait . The phenotype of this PA is also the opposite of swiggle, with a gain of function of its target resulting in a loss of function phenotype. Together, these studies thus demonstrate the broad applicability of PA technology to the dissection of cell biology.
The increase in MT1-MMP mediated cell migration and TR-gelatin degradation mediated by GFP-swiggle most probably results from the inhibition of endocytosis leading to increased levels of the protease at the cell surface. However, ICD mutants used in other studies that resulted in increased levels of MT1-MMP protein at the cell surface did not cause any effect on cell migration/invasion [30, 31, 33]. For example, over-expression of wild type MT1-MMP leads to increased cell migration and invasion, but truncations of the ICD including the LLY573 residues, or removal of the whole of the ICD prevented these effects [30, 31, 33]. This appears paradoxical as truncations that decrease the rate of MT1-MMP internalization should result in more MT1-MMP being present at the cell surface, and hence more proteolytic/migratory activity. One possible explanation for this observation is that the LLY573 motif of the MT1-MMP ICD is required both for clathrin-mediated internalisation and MT1-MMP-mediated cell migration. However, no tools previously existed to test this idea. Our results now show that the expression of GFP-swiggle reduces the rate of internalization of MT1-MMP and results in an increased amount of MT1-MMP at the cell surface, mimicking this aspect of LLY573 mutation. But unlike the ICD LLY573 mutation, GFP-swiggle also allows cell migration to proceed.
A potential caveat of our experiments as well as those of [30, 31, 33], is that the majority of the assays that were used were only sensitive to over-expressed levels of MT1-MMP. While this may reflect the pathological situation in cancer cells, further work is needed to assess whether GFP-swiggle can perturb other functions of MT1-MMP in normal cells through the development of assays that are sensitive to endogenous levels of MT1-MMP.
In summary, we have identified and characterized a PA that, when expressed in mammalian cells, can inhibit the clathrin-mediated internalisation of MT1-MMP by interacting with the normal function of the ICD of the protease. In addition to laying the ground-work for the study of the mechanism of endocytic recycling of transmembrane MMPs, this PA should be a useful tool in further studies of MT1-MMP in the existing wide range of biological and disease models.
All chemicals were AnalaR grade and were purchased from Sigma Aldrich Chemical Co. (Poole, UK) unless indicated otherwise.
Oligonucleotides coding for a PGGG linker followed by MT1-MMP, MT2-MMP, MT3-MMP, or MT5-MMP intracellular domain (ICD) were annealed and cloned downstream of the LexA DNA binding domain (DBD) in pEG202 (Origene, Rockville, USA) to generate LexA-MT1, -MT2, -MT3 and -MT5. The MT1-MMP ICD LLY/A mutant in pEG202 was created by site-directed mutagenesis (Stratagene, La Jolla, CA, USA). Swiggle, and s14, all in pJG4-5 (Origene), were amplified by PCR and subcloned into pIRES2eGFP (BD Clontech, UK). Swiggle and s14 were excised from the above constructs and subcloned into BretGFP-C1 (PerkinElmer, Boston, MA, USA). TrxA, excised from pJM-1 (gift from R. Brent, Berkeley, USA) was subcloned into BretGFP-C2. N-terminally His6-tagged swiggle was generated by cloning swiggle into pET32a (Merck Biosciences Ltd., Nottingham, UK).
Yeast two-hybrid screen
The peptide aptamer (PA) library containing a B42 AD fusion to 1 × 106 different PAs was constructed as described in  and transformed into the reporter S. cerevisiae strain EGY48 (MATα leu2::LexA 6op-LEU2 his3 trp1 ura3) . These cells were mated with EGY42 cells (MATa leu2 his3 trp1 ura3) ) carrying LexA-MT1. The two-hybrid mating screen was performed essentially as described by . Interactors, selected on Ura-His-Trp-Leu-/X-Gal, Gal, Raff plates after four days at 30°C, were picked and the plasmids rescued into E. coli KC8 cells. The plasmids were transformed back into EGY48 to confirm interaction with LexA-MT1 in EGY42 using an interaction-mating matrix. LexA-Cdk4 and AR cDNAs were kindly provided by P. Hinds and M. Lu (Harvard Medical School, USA). AD-CyclinD1 cDNA was from R. Brent (Molecular Sciences Institute, Berkeley CA, USA).
Isolation of mutants of swiggle by PCR mutagenesis of the swiggle peptide insert
PCR mutagenesis of the swiggle peptide insert was performed as previously described  except for the following alterations. Mutated swiggle sequences were ligated into Rsr II-cut pJM-1 vector and were transformed into XL-10 gold E. coli cells (Invitrogen Ltd., Paisley, UK). Plasmid DNA was isolated using Qiagen midi-prep DNA extraction kit (Qiagen, Crawley, UK), and transformed (1 μg) into EGY48 containing LexA-MT1 and pJK103 . Positive (blue) and negative (white) interactors were selected on Ura-His-Trp-Leu-/X-Gal, Gal, Raff plates for four days, picked and the plasmids rescued into E. coli KC8 cells. Plasmids were transformed back into EGY48 to confirm interaction with LexA-MT1 in EGY42 using an interaction-mating matrix.
Cell culture and transfections
All cell culture reagents were purchased from Invitrogen Ltd. unless indicated. MCF7 cells, purchased from ECACC (Salisbury, UK), were maintained in DMEM, supplemented with 10% (vol/vol) fetal calf serum (FCS, Hyclone Laboratories, UT, USA) and 2 mM L-glutamine at 37°C in 5% CO2 atmosphere. Transfections were performed using FuGENE-6 reagent (Roche Diagnostics, Lewes, UK) according to manufacturer's instructions.
Immunoprecipitation and western blotting
MCF7 cells were transfected for 48 hours with either 0.4 μg GFP-swiggle and 0.6 μg MT1-MMP constructs, or 0.4 μg GFP-TrxA and 0.6 μg MT1-MMP in 6-well plates. After 2 washes with ice-cold PBS, cells were lysed for 15 minutes on ice under rocking conditions in 200 μl of lysis buffer (50 mM Tris-HCl, pH 7.4, 100 mM NaCl, 2 mM EDTA, 1% Triton X-100, 60 mM octyl-D-glucoside) supplemented with complete protease inhibitor cocktail (Roche Molecular Biochemical, Hertfordshire, UK), sonicated (80 Volts for 10 seconds, Sonics and Material Inc., Suffolk, UK) and centrifugated (10 minutes at 15000 g at 4°C). An aliquot (10%) of the protein extract was kept and analysed by Western blot (input lysate). The remaining protein extract was incubated for 2 hours at 4°C with an anti-GFP rabbit polyclonal serum (dilution 1/2200). Protein G sepharose beads (20 μl of slurry) were then added and the mixture rotated for a further 2 hours at 4°C. Beads were washed four times with 1 × PBS, once with PBS diluted 5 times in distilled water and finally were resuspended in Laemmli sample buffer (LSB). Immunoprecipitates and input lysates were boiled for 5 minutes, resolved on 12% SDS-polyacrylamide gels, and electrotransferred onto PVDF membrane (Millipore, Watford, UK). Western blots were carried out as previously described . Rabbit anti-GFP serum (Abcam, Cambridge, UK) was used at a 1:2000 dilution. The anti-MT1-MMP sheep N175 pAb, directed against the entire extracellular of the protease [49, 50], was used at 10 μg/ml.
Cell surface biotinylation of proteins
Cell surface biotinylation was carried out as previously described , except for the following alterations. MCF7 cells (1 × 105) were transfected with 0.4 μg GFP-swiggle and 0.6 μg MT1-MMP, 0.4 μg GFP-TrxA and 0.6 μg MT1-MMP, 0.4 μg GFP-s14 and 0.6 μg MT1-MMP, or 0.4 μg GFP and 0.6 μg MT1-MMP in 6-well plates. PBS buffer was substituted for Soerensen buffer (SBS). Cells were incubated for 30 minutes in ice-cold PBS containing 0.5 mg/ml of NHS-SS-Biotin (Pierce Biochemical, Rockford, USA) and lyzed for 15 minutes at 4°C in 200 μl ice-cold RIPA buffer containing protease inhibitor III cocktail (Calbiochem Biochemical, West Drayton, UK). The lysate was cleared by centrifugation (16000 g for 5 minutes at 4°C) and incubation with protein A/G plus-agarose beads (25 μl; Santa Cruz Biotechnology, Santa Cruz, USA) for 1 hour at 4°C. An aliquot of the whole cell lysate (20 μl; input lysate) was kept for western blot analysis. Anti-biotin mAb (12 μg; Jackson Immunoresearch Ltd., Soham, UK) and Protein A/G Plus agarose beads (30 μl) were then added to the extract and incubated for 1 hour at 4°C under constant rotation. Beads were washed five times with ice-cold RIPA buffer, resuspended in LSB and processed for western blotting as previously described.
Cells seeded on glass coverslips were washed in PBS, fixed at room temperature (RT) in 4% (wt/vol) paraformaldehyde (PFA, BDH, Poole, UK) for 5 minutes and washed twice with PBS. For permeabilized cells, coverslips were incubated in 0.2% Triton-X100 in PBS for 5 minutes at RT, and washed three times in 2 × PBS. Cells were blocked for 30 minutes at RT with PBS containing 10% FCS and 10 μg/ml BSA, and then incubated with the anti-MT1-MMP sheep pAb (10 μg/ml) for 16 hours at 4°C in PBS containing 10 μg/ml BSA. After 3 washes in PBS, cells were incubated for 1 hour at RT with fluorescently-conjugated secondary antibody (Jackson Immunoresearch Ltd.) in PBS and according to the manufacturer's instructions. Coverslips were washed repeatedly in PBS, and mounted onto glass slides using Vectashield containing diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, USA). Images of fluorescently labelled cells were collected using a Zeiss LSM510 Metaconfocal microscope in a single focal plane (Carl Zeiss Ltd., Welwyn Garden City, UK).
Texas Red-labelled Gelatin Degradation Assay
Texas Red labelled-gelatin was coated, as previously described by  on the glass surface of 8 well Labtek culture slides (Becton Dickinson Labware, USA) and incubated for 2 hours at 37°C in DMEM containing 10% FCS. Untransfected MCF7 cells (5 × 104 per well) were then seeded on the fluorescent gelatin and incubated for 16 hours at 37°C. Cell transfections were carried out using FuGENE-6 (0.75 μl) and 250 ng of DNA (ratio used). Cells were then washed in warmed PBS, fixed with 4% (w/v) PFA in PBS and then processed immunofluorescence microscopy using the anti-MT1-MMP sheep pAb as described above. A Cy5 conjugated donkey anti-sheep secondary antibody (1:200 dilution) was used (Jackson Immunoresearch).
For each condition, pictures of at least 50 cells in a single focal plane were taken with a Zeiss 510 Meta confocal microscope. The area of TR-gelatin degradation was then measured with Zeiss AIM software (version 3.2).
Phagokinetic track colloidal gold cell migration assay
The phagokinetic track colloidal gold cell migration assay was performed as described previously . Colloidal gold-coated coverslips were placed in a 12-well plate, and transfected MCF7 cells were seeded at 2 × 103 cells per well. After 24 hours incubation at 37°C, cells were fixed as previously described and phagokinetic tracks were visualized using bright field illumination with a Zeiss 510 Meta confocal microscope. The area of migration for at least 50 transfected cells was measured with Zeiss AIM software and averaged.
MT1-MMP antibody internalization assay
MCF7 cells, co-transfected with GFP-swiggle (0.4 μg) and MT1-MMP (0.6 μg), or GFP-s14 (0.4 μg) and MT1-MMP (0.6 μg), were seeded on glass coverslips in 6-well plates. After 48 hours transfection, cells were washed twice with ice-cold PBS and incubated with the affinity purified N175 anti-MT1-MMP sheep pAb (5 μg/ml) at 4°C for 2 hours. Coverslips were then washed twice with ice-cold PBS to remove unbound antibody and fixed immediately (zero minute timepoint) in 4% PFA for 10 minutes or placed at 37°C for 10, 30 and 50 minutes in prewarmed media before fixation. Cells were permeabilized and processed for immuno-fluorescence microscopy as previously described. Endocytosis was quantified by measuring the fluorescence intensity of all endocytic vesicles per cell using Metamorph imaging software version 6.1 (Molecular Devices Ltd., Wokingham, UK) as previously described by . At least 5 cells were used for time 0 and 15 cells for the other time points.
membrane-type I matrix metalloproteinase
We would like to thank Gillian Murphy, Ron Laskey, Sharon Tate, Vihandha Wickramasinghe and Anasuya Chattopadhyay for their advice and support. We thank Yoshifumi Itoh (Kennedy Institute of Rheumatology) for his help and advice with the colloidal gold assay. We also thank Neil Taylor, William English and Sue Atkinson for critical reading of this manuscript. We would like to acknowledge the support of the Gates Cambridge Trust (RDW), the Medical Research Council (RDW and PKF), Cancer Research UK and Hutchison Whampoa Limited (CR) and the University of Cambridge.
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