Maged1, a new regulator of skeletal myogenic differentiation and muscle regeneration
© Nguyen et al; licensee BioMed Central Ltd. 2010
Received: 10 May 2010
Accepted: 20 July 2010
Published: 20 July 2010
In normal adult skeletal muscle, cell turnover is very slow. However, after an acute lesion or in chronic pathological conditions, such as primary myopathies, muscle stem cells, called satellite cells, are induced to proliferate, then withdraw definitively from the cell cycle and fuse to reconstitute functional myofibers.
We show that Maged1 is expressed at very low levels in normal adult muscle but is strongly induced after injury, during the early phase of myoblast differentiation. By comparing in vitro differentiation of myoblasts derived from wild-type or Maged1 knockout mice, we observed that Maged1 deficiency results in reduced levels of p21CIP1/WAF1, defective cell cycle exit and impaired myotube maturation. In vivo, this defect results in delayed regeneration of injured muscle.
These data demonstrate for the first time that Maged1 is an important factor required for proper skeletal myoblast differentiation and muscle healing.
Skeletal muscle contains quiescent mononucleated myogenic cells, called satellite cells, that are able to proliferate in response to injury and give rise to new functional myofibers. Soon after a lesion, inflammatory cells such as neutrophiles and macrophages are recruited at the damaged site where they release growth factors and cytokines that induce satellite cells to proliferate. The activated satellite cells divide asymmetrically to (1) reconstitute the stock of stem cells and (2) provide myoblasts that will first proliferate and then undergo myogenic differentiation and maturation to form new muscle fibers. The new cells can fuse between themselves or with existing fibers. Muscle differentiation involves a cascade of muscle-specific gene activation coordinated with irreversible withdrawal from the cell cycle. The commitment of cells to the muscle lineage and to progression through differentiation requires the activation of a limited set of muscle-specific transcription factors. These key factors are the basic helix-loop-helix (bHLH) transcription factors MyoD, Myf5, myogenin (MyoG) and MRF4, which are collectively called myogenic regulatory factors (MRFs). The MRFs activate their target genes by binding to the E-box CANNTG consensus sequence [1–4]. Primary cultures of newborn or adult skeletal muscles or established muscle cell lines like C2C12 can be used to study myogenic differentiation. These cells can be maintained as dividing cells in serum rich medium (GM), but under the condition of mitogen deprivation, they exit the cell cycle in G1 to G0, express the repertoire of muscle-specific genes and fuse to form syncytial myotubes [5, 6]. MyoD is expressed early after satellite cell activation. In dividing cells, its function is repressed, but upon induction of differentiation, its transcriptional activity is induced, and it governs two distinct essential functions: (1) activation of muscle specific genes, such as MyoG, and (2) induction of withdrawal from the cell cycle by activation of the cell cycle inhibitors p21CIP1/WAF1 and Rb [7, 8].
Necdin, a member of the large family of Mage proteins, has recently been reported to promote myoblast survival and differentiation and to improve skeletal muscle regeneration [9, 10]. Necdin has also been shown to protect muscle fibers from tumor-induced wasting by interfering with the TNF signaling cascade . Maged1 (also called Nrage and Dlxin1) is an X-linked Mage family member that physically interacts with Necdin . Although in vitro studies have suggested a role for Maged1 in diverse biological functions, only its role in the apoptotic signaling cascade emanating for the TNF receptor superfamily member p75NTR (p75 NeuroTrophin Receptor) has been confirmed in vivo so far [13–15]. Interestingly, data obtained by high throughput expression analysis indicate that the expression of MAGED1 RNA is upregulated in the muscles of Duchenne muscular dystrophy (DMD) patients, a condition in which muscle regeneration concomitantly occurs with the degeneration of myofibers . This observation and the fact that Maged1 physically interacts with Necdin encouraged us to investigate the possible roles of Maged1 in muscle regeneration and differentiation. Here, we show that Maged1 is transcriptionally upregulated during myogenic differentiation and that Maged1 deficiency results in deficient myoblast differentiation and maturation, leading to delayed muscle regeneration in vivo.
Maged1 is induced early during myogenic differentiation
Maged1 transcription is activated by MRFs during myoblast differentiation
To test this hypothesis, the DNA fragment from -1977 to +80 of the human MAGED1 promoter was inserted upstream of the firefly luciferase reporter gene and tested for cis-activation in proliferating and differentiating C2C12 myoblasts. We found that the luciferase activity controlled by this fragment increased approximately ten fold 24 hours after the induction of differentiation by serum starvation. To localize the key regulatory elements in this fragment, we deleted segments from its 5' end and found that the -531+80 fragment had nearly the same activity as the -1977+80 fragment (Figure 2A-B). This indicates that the two proximal E-boxes at -227 and -118 (especially the box at -118, which is conserved in mammals) are possible important sites for transcriptional induction. We tested the importance of these two E-boxes by targeted mutagenesis. Mutation of the -118 E-box resulted in a significant reduction of induction after serum starvation, indicating that this site is important for induction and that MRFs are probably directly involved (Figure 2C). We then tested the potential of transfected MyoD to transactivate the -531+80 fragment in NIH-3T3 cells, which do not express endogenous MyoD. MyoD induced a three-fold increase in the expression of the luciferase reporter (Figure 2D and Additional file 2: Figure S2). Altogether, these results show that the MAGED1 promoter is activated upon the induction of myoblast differentiation and contains conserved E-boxes that are important for its transactivation by MyoD.
Deficiency of Maged1 results in impaired myogenic differentiation
Maged1 is required for normal p21CIP1/WAF1 expression and cell cycle withdrawal of differentiating myoblasts
Discussion and Conclusions
Skeletal muscle satellite cells are quiescent stem cells that are activated in response to physiological or pathological stimuli such as after muscle injury or in chronic pathological conditions. After an acute lesion, the initial phase of muscle repair is characterized by inflammation and degeneration of the damaged tissue. Almost simultaneously, satellite cells are activated and proliferate to generate a population of myoblasts that differentiate and fuse to form new multinucleated myotubes. Muscle regeneration is a tightly orchestrated process, and understanding the molecular and cellular mechanisms responsible for its control is important for the development of treatments of muscles impaired by aging, disease or atrophy.
Maged1 is upregulated during myoblast differentiation and muscle regeneration
Maged1 is silent in normal adult muscles, but it is massively induced soon after injury of skeletal muscles. In primary cultures of myoblasts, Maged1 is expressed in proliferating cells, and its expression increases within the first 24 hours after the induction of terminal differentiation at the time when cells normally exit from the cell cycle. We observed that high levels of Maged1 RNA and protein were maintained as long as the differentiation proceeded. Promoter analysis showed that Maged1 transcription was upregulated during myogenic differentiation and that two E-boxes present in the proximal promoter were responsible for this activation. This strongly suggests that MyoD and/or other MRFs is (are) responsible for this regulation.
Maged1 is required for efficient myoblast cell cycle withdrawal, myogenic differentiation and normal muscle regeneration after injury
The induction of Maged1 during muscle differentiation and regeneration suggested that it could play a role during these processes. We showed that mouse primary myoblasts deficient in Maged1 had a largely reduced capacity to differentiate and to fuse. In vivo, the loss of Maged1 leads to delayed regeneration after muscle injury with the production of smaller muscle fibers. This deficit appears to result from a late block in the differentiation program. Indeed, in the absence of Maged1, we observed normal expression of MyoD and MyoG, which are early differentiation markers expressed in proliferating cells, whereas the late markers eMHC (a muscle structural gene) and MCK (an important muscle kinase) were not induced properly. The mechanism responsible for this deficit remains to be fully elucidated, but the reduced ability of the cells to establish the G0 arrest probably contributes to the impaired differentiation. A similar observation was made in P19 embryonic carcinoma cells differentiating in neurons after treatment with retinoic acid: downregulation of Maged1 by morpholinos disturbed neurogenesis by impairing cell cycle withdrawal . Maged1 thus appears to be a key component regulating the transition of progenitors to differentiated neurons and muscle cells by interfering with the cell cycle progression.
A future direction of inquiry following these results will be to determine how Maged1 regulates the G1 to G0 transition in differentiating myoblasts. This cell cycle arrest requires the retinoblastoma tumor suppressor (Rb) protein [23, 24]. The precise mechanisms leading to the activation of Rb during myogenesis remain unclear. Rb activity is controlled by its phosphorylation by cyclin-dependent kinases (Cdk), which are in turn controlled by Cdk inhibitors, including p21CIP1/WAF1 . In differentiating myoblasts, p21CIP1/WAF1 transcription is directly controlled by members of the p53 family . Indeed, ectopic expression of ΔNp73, a transdominant inhibitor of all p53 members (p53, p63 and p73) prevents p21CIP1/WAF1 induction, cell cycle withdrawal and myogenic differentiation . The fact that p53 knockout mice exhibit normal muscle development excludes an essential role for this protein in myogenic differentiation and points at p63 and/or p73 as the key factors in this process [26, 27]. Additionally, it has been shown that overexpression of Maged1 in HEK293 cells activates p53-dependent transactivation of the p21 CIP1/WAF1 promoter . As p21CIP1/WAF1 expression is reduced in Maged1-deficient myoblasts, we can hypothesize that Maged1 acts as a transcriptional cofactor of p63 and/or p73 to promote p21CIP1/WAF1 expression and cell cycle exit. Alternatively, Maged1 could act indirectly, by sequestering an inhibitor of p63 and/or p73 activity. Such a mechanism has been shown for Necdin, which sequesters EID-1, an E1A-like protein that inhibits MyoD by suppressing the acetyltransferase activity of its transcriptional co-activator p300 .
It has been shown previously that Maged1 can regulate transcription by interacting with several transcription factors and regulators [30–33]. Besides its involvement in the regulation of cell proliferation through p21CIP1/WAF1 expression, Maged1 could directly or indirectly regulate other genes essential for the skeletal myogenic differentiation. A role for Maged1 in cell adhesion has been reported by Xue et al. , we could therefore imagine that deficient cell adhesion and/or fusion could also participate in the observed muscle phenotype.
Maged1 is broadly expressed in embryonic and adult tissues and has been identified as interacting with many proteins implicated in apoptosis signaling, cell cycle regulation, cell adhesion and migration, cell differentiation and transcription regulation [12, 22, 28, 31, 34–41]. However, the physiological relevance of these interactions remains largely to be demonstrated. We have produced Maged1 knockout mice and have shown previously that Maged1 acts as a pro-apoptotic factor required for normal developmental apoptosis of sympathetic, sensory and motor neurons in vivo . Here, we show that Maged1 plays an important role in myogenic differentiation by allowing myoblasts to express appropriate levels of p21CIP1/WAF1, exit the cell cycle and undergo terminal differentiation.
Antibodies and chemicals
The following reagents were purchased as indicated: mouse anti-Nrage (cat. 612500) and mouse anti-BrdU (cat. 555627) from BD Sciences; rabbit anti-MHC from Santa Cruz (cat. Sc-20641); rabbit anti-laminin (cat. L9393) from Sigma-Aldrich; fluorescent secondary Alexa-conjugated antibodies from Invitrogen; horseradish peroxidase-conjugated secondary antibodies from Dako; cell culture media and sera from Lonza Inc.; Dual Luciferase Reporter Assay kit from Promega; Hoeschst 33342 (cat. H1399) and TOPRO®-3 (cat. T3605) from Invitrogen; Vectastain ABC kit from Vector Laboratories; other chemicals from Sigma-Aldrich.
Animals and primary myoblast cell culture
Mice with the conditional floxed allele (Maged1 tm1Urfm ) and the Cre-recombined (constitutive knockout) allele for Maged1 have been described previously . Maged1 tm1Urfm/HSA-Cre mice lacking Maged1 only in differentiating muscle cells were obtained by crossing mice with a floxed Maged1 allele (Maged1 tm1Urfm/tm1Urfm ) with transgenic mice specifically expressing Cre recombinase in differentiating myoblasts (Tg(ACTA1-cre)79Jme/J) that were obtained from the Jackson Laboratory . Animals were maintained and treated in compliance with the guidelines specified by the Belgian Ministry of Trade and Agriculture (agreement LA 1900056). Primary myoblasts were isolated from the adult tibialis anterior (TA) muscle of two- to three-month old wild-type or constitutive Maged1 knockout mice following the protocol of the Springer Lab (University of California, San Francisco), which was adapted from the protocol of Springer, M.L., T. Rando, and H.M. Blau (1997) « Gene delivery to muscle » in Current Protocols in Human Genetics (John Wiley & Sons, New York). Cells were grown on collagen-coated plates, either in proliferation medium (Ham's F-10 supplemented with 20% FBS, 2.5 ng/ml bFGF, and 50 μg/ml gentamycin) or in differentiation medium (DMEM supplemented with 5% horse serum, 50 μg/ml gentamycin).
Histology and morphometry
TA muscles of Maged1 tm1Urfm/HSA-Cre and Maged1 tm1Urfm adult mice (2-4 months) were injected with 50 μl of 10 μM cardiotoxin (CTX) (from Naja mossambica mossambica, C9759 - Sigma-Aldrich) and harvested at the indicated time points. Muscles were fixed in Bouin's solution (for hematoxylin and eosin) or in 4% buffered paraformaldehyde solution (for immunostaining) and were paraffinized following standard protocol. For histological analysis, 6-μm serial sections were stained with hematoxylin and eosin according to standard protocol. To calculate the cross-sectional area of myofibers (XSA), three images were captured from each section and the surface of at least 800 myofibers per genotype per time point were measured using MATLAB (The MathWorks). Laminin immunostaining was performed on 6-μm sections following the instructions of the manufacturer. Nuclei were visualized by counterstaining with Hoeschst 33342.
Cell lines and plasmids
C2C12 myoblasts were a gift from Professor Raymackers (UCL, Louvain-la-Neuve, Belgium). C2C12 cells were maintained in DMEM supplemented with 20% FBS, 50 μg/ml gentamicin, and a 1x mixture of non-essential amino acids (growth medium, GM). Cells were induced to differentiate in DMEM supplemented with 2% horse serum and 50 μg/ml gentamicin (differentiation medium, DM). NIH-3T3 fibroblasts were cultured in DMEM supplemented with 10% FBS and 50 μg/ml gentamicin.
The MyoD expression plasmid pEMSV-MyoD (original researcher April Hawkins) was a kind gift of Eric N. Olson. Fragments of the human MAGED1 promoter and their mutated versions were amplified by PCR from human DNA and cloned into a pGL3-basic vector (Promega). Site-directed mutagenesis was performed using a triple PCR method. The primers used were the following: -1977 forward: 5'-TCAGAGCTCTATGAAGGAATCGGTTTGGGA-3'; -971 forward: 5'-TCAGAGCTCTTAGTCCCCAGGCCAGCATGA-3'; -531 forward: 5'-TCAGAGCTCTCCTCTCAGGCCATTCCGTT-3'; +80 reverse: 5'-TCAACGCGTGAGGATCGGGATTGCTGGGC-3'; Δ-118 forward: 5'-TCCAGCGCACA C G A GCATCATGGTCT-3'; Δ-118 reverse: 5'-AGACCATGATGCT C G TGTG CGCTGGA-3'; Δ-227 forward: 5'-GCACAGCGT ATC C G TC GGGGAGG-3'; Δ-227 reverse: 5'-CCTCCCCG AC G GAT A CGCTGTGC-3'. Italic sequences indicate E-boxes and letters in bold indicate mutated nucleotides.
Total RNA from cells from 6-cm plates was extracted using the RNeasy mini kit (Qiagen) and subjected to reverse transcription using the Impromt-II transcriptase (Promega) according to the manufacturer's instructions. Quantitative PCR was performed on 7.5 ng of cDNA in a volume of 20 μl with Power SYBR Green PCR Master Mix (Applied Biosystems) using the ABI 7300 real-time PCR System (Applied Biosystems). Unless otherwise cited, primer sequences were designed using Primer Express software (Applied Biosystems).
The primers used were the following: GAPDH forward: 5'-CGTGCCGCCTGGAGAA-3'; GAPDH reverse: 5'-GATGCCTGCTTCACCACCTT-3'; Maged1 forward: 5'-CAAGAGGACCCGCAAGGTT-3'; Maged1 reverse: 5'-GCCTTTGATCCCCACTGTTG-3'; MyoD forward: 5'-GCTGCCTTCTACGCACCTG-3'; MyoD reverse: 5'-GCCGCTGTAATCCATCATGC-3' ; MyoG forward: 5'-CCTGGAAGAAAAGGGACTGG-3'; MyoG reverse: 5'-TCATTCACTTTCTTGAGCCTGC-3' ; Mef2C forward: 5'-TGGAGAAGCAGAAAGGCACT-3'; Mef2C reverse: 5'-ATTCGTTCCCTCTGCACTTCT-3'; p21 forward: 5'-GGTGGTGGAGACCTGATGAT-3'; p21 reverse: 5'-GCACCTTTTATTCTGCTGGC-3'; eMHC forward: 5'-TGAAGAAGGAGCAGGACAC-CAG-3'; eMHC reverse: 5'-CACTTGGAGTTTATCCACCAGATCC-3' . MCK forward: 5'-GATTCTCACTCGCCTTCGTC-3'; MCK reverse: 5'-GCCCTTTTCCAGCTTCTTCT-3' .
5x104 NIH-3T3 fibroblasts or C2C12 myoblasts were seeded into each well of 24-well plates and transfected with 1 μg of DNA mixtures, including 20 ng of the Renilla luciferase-encoding plasmid pRL-CMV using ExGen500 and Turbofect reagent (Fermentas, Germany), respectively. To determine the activity of MAGED1 during differentiation in C2C12 myoblasts, 980 ng of pMAGED1-1977+80-luc, pMAGED1-971+80-luc or pMAGED1-531+80-luc plasmids were used for each transfection. To analyze the effect of MyoD on the activity of MAGED1 promoter in 3T3 fibroblasts, increasing amounts of the MyoD expression plasmid pEMSV-MyoD were cotransfected with 380 ng of pMAGED1-531+80-luc plasmid. Empty vector (pEMSV) was used as a substitute for pEMSV-MyoD if needed to maintain a constant total amount of transfected DNA. In experiments with mutated MAGED1 promoters in fibroblasts, 600 ng of pEMSV-MyoD or pEMSV were cotransfected with 380 ng of pMAGED1-531+80-luc plasmid or its mutated versions. Cells were harvested at the indicated time points and subjected to luciferase measurement using the Dual Luciferase Reporter Assay kit (Promega). Firefly luciferase activities were normalized to Renilla luciferase activities.
FACS and cell cycle analysis
Cells were harvested by trypsinization, fixed in 70% ethanol overnight at -20°C and incubated with propidium iodide (PI) solution (PI 10 μg/ml, sodium citrate 1.1% and RNAse A 1 mg/ml) for 30 minutes at 37°C before FACS analysis. For each sample, 10,000 cells were analyzed for DNA content in the FL2 channel using FACSCalibur platform (BD Sciences).
Immunocytochemistry and fusion index
First, 5x104 primary myoblasts were seeded in each well of 24-well collagen-coated plates in GM. Twenty-four hours later, cells were shifted to DM. To perform immunocytochemistry, cells were fixed in 4% buffered paraformaldehyde solution and incubated with anti-MHC for two hours at room temperature. MHC-expressing cells were visualized using the Vectastain ABC kit (Vector Laboratories). Labeled cells were photographed under a light microscope, and more than 800 nuclei per genotype per time point were counted. The fusion index was calculated by dividing the number of nuclei in MHC-expressing cells containing at least two nuclei by the total number of nuclei of MHC-expressing cells.
BrdU incorporation test
Cells were incubated for 2 hours with BrdU-containing medium (10 μM) before staining with anti-BrdU and secondary fluorescent antibodies following standard protocol. Nuclei were visualized by counterstaining with TOPRO®-3. Approximately 400 cells were counted under fluorescent microscopy to determine the percentage of labeled cells.
Protein extraction and immunoblot analysis
Cells and muscle tissues were homogenized in RIPA buffer containing 50 mM Tris-HCl pH 7.4, 1% NP-40, 0.25% Na-deoxycholate, 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 1 mM Na3VO4, and a protease inhibitor cocktail (Complete; Roche Diagnostics) and centrifuged at 1,000 g for 10 min at 4°C to discard cellular debris. The protein concentration was measured using the Pierce® BCA Protein Assay Kit. Sample preparation and western blot analysis were performed following standard protocol. Chemiluminescent signals were quantified by digital imaging using the Imagequant 350 system (General Electric)."
All experiments were performed at least three times and results were expressed as mean ± the standard deviation (SD). Statistical analyses were conducted using Student's t-test and the difference was considered significant when the probability value was < 0.05.
We thank Jean-Marc Raymackers for helpful discussions and Phil Barker for the generous gift of Maged1 antibody. We are grateful to Dominique Desnoeck and Daniel Van Vlaender for technical assistance and to Vincent Nicolas, Jonathan Ornan de Xivry, and Guillaume Janssens for help in muscle image analysis and XSA calculation. This work was supported by the Fonds de la Recherche Scientifique et Médicale (grant n° 3.4527.04) of the Fonds National de la Recherche Scientifique of Belgium (to ODB). HNTN was supported by the Commission Universitaire pour le Développement of the Communauté Française de Belgique. MB is a Fonds Wetenschappelijk Onderzoek (FWO) postdoctoral fellow.
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