Incomplete posttranslational prohormone modifications in hyperactive neuroendocrine cells
© Strating and Martens; licensee BioMed Central Ltd. 2009
Received: 15 October 2008
Accepted: 07 May 2009
Published: 07 May 2009
In black-background-adapted Xenopus laevis, the intermediate pituitary melanotrope cells are hyperactive, producing large amounts of their major secretory cargo proopiomelanocortin (POMC, representing ~80% of all newly synthesised proteins), whereas in white-adapted frogs these cells are only basally active. Here we explored in the hyperactive and basally active melanotrope cells the capacity for posttranslational POMC processing events in the secretory pathway.
We found that the hyperactive cells produced mainly non-complex N-glycosylated POMC, whereas in the basally active cells POMC was mostly complex N-glycosylated. Furthermore, the relative level of POMC sulphation was ~5.5-fold lower in the hyperactive than in the basally active cells. When the cargo load in the secretory pathway of the hyperactive cells was pharmacologically reduced, the relative amount of complex glycosylated POMC markedly increased.
Collectively, our data show that the secretory pathway in hyperactive neuroendocrine secretory cells lacks the capacity to fully comply with the high demands for complex glycosylation and sulphation of the overload of secretory cargo. Thus, a hyperactive secretory cell may run short in providing an output of correctly modified biological signals.
A variety of vertebrate secretory cells with a basal activity become highly activated during their development or maturation, such as the immunoglobulin-secreting plasma cells and the insulin-secreting pancreatic beta-cells. Induction of cells to become professional secretors involves massive changes at the level of gene transcription, protein biosynthesis and cellular ultrastructure. Secretory organelles such as the endoplasmic reticulum, the Golgi apparatus and the secretory granules become expanded and a variety of genes is upregulated, including those encoding the secretory cargo and the machinery involved in the transport and biosynthesis of the cargo (see for example [1, 2]). We use a unique neuroendocrine cell model, namely the Xenopus laevis intermediate pituitary melanotrope cells, to study the events occurring during the activation of neuroendocrine cells to become professional secretory cells. The melanotropes are inducible neuroendocrine cells that play a central role in the process of background adaptation of the animal. When the frogs are on a white background (white adapted; WA), the melanotrope cells are only basally active. Placing the animals on a black background (black adapted; BA) physiologically activates the melanotrope cells to become hyperactive professional secretory cells. Hyperactive melanotropes are dedicated to produce and proteolytically process vast amounts of the prohormone proopiomelanocortin (POMC) and secrete the POMC-derived products (reviewed in ). In Xenopus melanotrope cells, POMC is synthesised as an N-glycosylated and sulphated 37 K prohormone [4, 5] that is subsequently cleaved to generate a number of bioactive peptides. The C-terminal portion of POMC is processed to α-melanophore-stimulating hormone (MSH), β-MSH, corticotropin-like intermediate lobe peptide (CLIP), β-endorphin and some linking regions of unknown function [4, 6, 7]. The N-terminal part of POMC (18 K POMC), which bears the only N-glycan present in Xenopus POMC, can be cleaved to two different γ-MSH peptides, although the majority (90–95%) is not processed to γ-MSH but secreted as glycosylated 9 K or 18 K products . MSHs released into the blood cause darkening of the skin to allow the animal to adapt to its background.
When considering neuroendocrine cell activation one wonders whether basally active secretory cells that are physiologically activated do not only change the quantity but also the quality of their secretory output. A change in output quality would imply an involvement of the machinery responsible for the biosynthesis of the secretory cargo. In this study we tested this hypothesis by exploring the biosynthetic machinery in the basally active and hyperactive Xenopus melanotrope cells, in particular the implications of secretory cell induction of the Golgi-based posttranslational processing of secretory cargo proteins. We find profound differences in POMC N-glycosylation and sulphation between the hyperactive and the basally active melanotrope cells. Our experiments indicate that in the hyperactive melanotropes the Golgi apparatus lacks sufficient glycosylation and sulphation capacity to properly process the high amounts of newly synthesised POMC.
Biosynthesis of 18 K and 18 K* POMC in the melanotrope cells of black- and white-adapted Xenopus
Dynamics in the biosynthesis of 18 K and 18 K* POMC in the melanotrope cells of black-adapted Xenopus
Glycosylation of 18 K and 18 K* POMC in the melanotrope cells of black- and white-adapted Xenopus
To examine the difference in the glycogroups in more detail, we deglycosylated the newly synthesised proteins with Endoglycosidase H (EndoH), an enzyme that selectively removes high-mannose N-glycans. We observed that in BA NILs 37 K POMC was EndoH-sensitive (Figure 3A), suggesting that it had not yet undergone complex glycosylation. Since the proteolytic cleavage of 37 K POMC starts already in the trans-Golgi network (TGN) and continues in the TGN-derived immature secretory granules , 37 K POMC is proteolytically processed soon after it has encountered the Golgi-enzymes that process the N-glycans to EndoH-resistant conformations. Therefore, the majority of the EndoH-sensitive 37 K POMC molecules may have resided in a pre-Golgi compartment and may not yet have encountered the enzymes that confer EndoH-resistance. To prevent the proteolytic processing of 37 K POMC and thus cause the prohormone to accumulate in a post-Golgi compartment, we performed pulse-chase experiments with BA NILs in the presence of bafilomycin, a drug that inhibits acidification of the TGN/secretory granules. Surprisingly, in the presence of bafilomycin the amount of EndoH-resistant 37 K POMC increased only marginally (not shown). In line with this observation, the vast majority of the 18 K POMC processing product that was produced in the absence of bafilomycin was EndoH-sensitive as well (Figure 3A). These findings suggest that BA melanotropes are unable to convert the N-glycans on POMC to complex glycogroups. Remarkably, in WA melanotropes a significant portion of the 37 K and 18 K* POMC molecules was EndoH-resistant (Figure 3A) showing that, while the prohormone passes through the Golgi apparatus, the WA cells possess the capability to convert a substantial portion of the N-glycans of POMC to a complex form. Interestingly, a part of the 18 K* POMC molecules in WA cells was still EndoH-sensitive. Hence, 18 K* POMC does apparently not represent a single product but rather constitutes 18 K POMC products with slightly different N-glycans. Possibly, these differentially glycosylated 18 K* POMC products represent intermediates of high-mannose to complex glycosylated 18 K POMC products.
Sulphation of 37 K POMC in the melanotrope cells of black- and white-adapted Xenopus
In addition to N-glycosylation, POMC is also posttranslationally modified by sulphation. We therefore wondered whether POMC produced in BA and WA melanotropes differs not only in its glycosylation but also in its sulphation state. To determine the relative amount of POMC-sulphation, we pulse labelled the cells for 15 minutes simultaneously with [3H]lysine and [35S]sulphate, and determined the amount of each label incorporated into newly synthesised 37 K POMC. We observed that the relative amount of sulphated 37 K POMC was ~5.5-fold higher in WA than in BA cells (Figure 3B). Sulphate-modifications can be attached directly to the protein backbone through a tyrosine residue or indirectly through N-glycans.
Deglycosylation of [35S]sulphate labelled 37 K POMC with PNGaseF did not reduce its level (Figure 3C), indicating that Xenopus POMC is not glyco- but rather tyrosine-sulphated. The Sulfinator prediction program  indeed predicts a sulphation site on tyrosine residue 188 (SLELDY188PEIDLDEDIED) in the C-terminal half of Xenopus 37 K POMC.
Deglycosylation with EndoH revealed that essentially all sulphated POMC from BA cells was EndoH-sensitive, whereas ~50% of the sulphated POMC from WA cells was EndoH-resistant (Figure 3C). The ratio of EndoH-sensitive to -resistant [35S]sulphate-labelled 37 K POMC from WA cells is similar to that of [35S]methionine/cysteine-labelled 37 K POMC from WA cells (compare Figures 3A and 3C), suggesting that the sulphation machinery does not discriminate between complex and non-complex glycosylated 37 K POMC.
Biosynthesis of 18 K and 18 K* POMC in cycloheximide-treated melanotrope cells of black-adapted Xenopus
In this study, we set out to examine whether hyperactive neuroendocrine secretory cells are able to tune their secretory pathway such that they can comply with the extremely high demands for proper posttranslational modifications of the vast amounts of secretory cargo. We chose as a model cell system the physiologically inducible Xenopus melanotrope cells that produce POMC as their main secretory cargo. We show that the hyperactive BA melanotrope cells produced 18 K POMC as the major and 18 K* POMC as a minor POMC-derived product. In contrast, the basally active WA melanotropes produced almost exclusively 18 K* POMC. The difference between the 18 K and 18 K* POMC products concerned only the N-glycan moiety and not the protein backbone. The 18 K POMC product from WA and BA cells as well as most of the 18 K* POMC from BA cells was EndoH sensitive and thus non-complex glycosylated. Remarkably, a significant portion of the 18 K* POMC from WA cells was EndoH resistant and thus complex glycosylated. These findings show that the maturation of N-glycans on the POMC molecule depended on the biosynthetic activity of the cells, and was heterogeneous and incomplete (i.e., not processed to complex glycans), in particular in BA melanotropes. In addition, the extent of POMC tyrosine sulphation differed greatly in the BA and WA cells. Thus, the BA and WA melanotropes secrete a collection of differentially glycosylated and sulphated POMC-derived products. Interestingly, in BA melanotrope cells the rate of biosynthesis of 18 K* POMC was higher than that of 18 K POMC. In addition, 18 K* POMC was released into the incubation medium faster than 18 K POMC, presumably as a result of the faster biosynthesis of 18 K* POMC. The 18 K* POMC product from BA cells may well originate from a pool of 37 K POMC molecules travelling relatively fast through the secretory pathway (restricting the opportunity for post-translational modifications) or, alternatively, bypassing certain machinery-containing subcompartments of the secretory pathway. Clearly, the secretory pathways in the WA and BA melanotrope cells differently fulfil the needs for posttranslational prohormone processing.
The reduced degree of N-glycan processing and sulphation of POMC in BA melanotropes led us to hypothesise that in hyperactive melanotropes the Golgi apparatus is not equipped with sufficient machinery to fully complex glycosylate and sulphate cargo molecules. If true, a reduction in the amount of cargo passing through the Golgi apparatus should increase the fraction of complex glycosylated and sulphated POMC. To test this hypothesis, we chose to reduce POMC biosynthesis by cycloheximide, an inhibitor of protein biosynthesis. In the presence of increasing concentrations of cycloheximide, protein biosynthesis was indeed reduced, whereas the relative amount of complex glycosylated POMC had clearly increased. In summary, we show that in neuroendocrine Xenopus melanotrope cells that have been physiologically induced to hyperactive secretory cells the Golgi apparatus is not sufficiently equipped to allow full posttranslational processing of the high amounts of secretory cargo produced. As a result, the hyperactive Xenopus melanotropes produce a repertoire of POMC-derived products that are heterogeneously glycosylated and sulphated. The observed heterogeneous posttranslational modifications of secretory cargo do not appear to be restricted to the BA Xenopus melanotropes. For example, in bovine posterior pituitaries ~50% of the POMC-derived protein β-lipotropin is sulphated , only 5–10% of the N-terminal portion of Xenopus POMC is processed to γ-MSH peptides  and a 16 K POMC product (the N-terminal part of bovine POMC) is only partly O-glycosylated and cleaved . Unfortunately, these studies did not include an analysis of the effect of physiological cell activation on the posttranslational modifications of POMC.
Since previous studies that have examined the effect of the differential glycosylation and sulphation events on the biosynthesis, intracellular processing, biostability and secretion of POMC are contradictory and inconclusive [5, 14–21], the implications -if any- of the affected posttranslational modifications remain unclear, including their effect on the bioactivity of the POMC-derived products. Nevertheless, glycosylation and sulphation are known to alter biological properties, such as the stability and bioactivity of secretory cargo proteins. For example, in frogs the glycans on POMC may provide stability against non-specific proteolysis or degradation [19–21] and the bioactivity of the N-terminal portion of bovine and mouse POMC depends on (the extent of) N- and O-glycosylation [14, 22]. Also, the bioactivity of a variety of peptide hormones, e.g. gastrin, cholecystokinin, leu-enkephalin, prolactin, thyrotropin, gonadotropins and follicle stimulating hormone, is known to be influenced by posttranslational modifications [23–30]. Furthermore, a number of signalling pathways, such as the Notch signalling pathway and chemokine signalling and immune regulation pathways, depend on the proper posttranslational modifications of the extracellular signalling molecule or the receptor [31–35]. It is therefore conceivable that the differentially glycosylated and sulphated products secreted by the hyperactive and the basally active melanotropes vary in their bioactivities, and thus that the BA and WA cells have different secretory signals.
We show that the Golgi apparatus of the hyperactive melanotrope cells is apparently equipped with the appropriate machinery to complex glycosylate and sulphate POMC molecules, but the quantity of the machinery is not sufficient to fully process the vast amounts of POMC passing through the secretory pathway of BA melanotropes. The shortcomings in protein processing events that we have observed in the hyperactive Xenopus melanotrope cells may also occur in other hyperactive secretory cell systems, including in neuroendocrine tumour cells and immunoglobulin-secreting plasma cells, suggesting that our findings may well have broader implications.
Animals were bred and reared in the Central Animal Facility of the Radboud University (Nijmegen, The Netherlands). Animals were background adapted for at least three weeks. All animal experiments were carried out in accordance with the European Communities Council Directive 86/609/EEC for animal welfare and permits RU-DEC 2003–53 and 2007–027 from the animal experiment committee of the Radboud University for the use of Xenopus laevis.
Pulse and pulse-chase analysis
Metabolic cell labelling experiments with [35S]methionine/cysteine or with [3H]lysine and [35S]sulphate and quantification of the newly synthesised products were performed as described previously [10, 36]. The Xenopus pituitary consists of three parts; the anterior lobe, the intermediate lobe containing the melanotrope cells, and the neuronal lobe consisting of nerve endings originating from the hypothalamus. The anterior lobe can easily be dissected from the pituitary, but the intermediate and neuronal lobes (together the NIL) are intimately associated. Since the neuronal lobe is biosynthetically inactive, we used the NIL for our biosynthetic studies. Dissection of the NIL from WA animals disrupts the inhibitory action of hypothalamic neurons. One of the major factors of hypothalamic origin inhibiting peptide release from the melanotrope cells is dopamine . In order to retain an inhibitory action on peptide secretion during the in vitro incubations of the NILs from WA frogs, we added the dopamine receptor agonist apomorphine to the incubation medium. The removal of all N-linked glycogroups irrespective of their composition by Peptidyl N-glycosidase F was performed as described . For the selective removal of high-mannose glycogroups, protein homogenates were boiled in 85 mM sodium acetate pH 5.5/0.04% SDS/0.08% β-mercaptoethanol/1 mM phenylmethylsulfonyl fluoride (PMSF) for 10 min, cooled to room temperature, 1 μl 5% Triton X-100 and 5 mU Endoglycosidase H (EndoH; Roche) were added, and the samples were incubated overnight at 37°C.
Statistical evaluation was performed using unpaired two-tailed t-tests. In those cases where the variances were significantly different, Welch's correction was used. Differences in mean values were considered of statistical significance if p < 0.05. Calculations were performed using the GraphPad Prism 4 program (GraphPad Software).
We thank Ron Engels for animal care and the members of the Martens laboratory for helpful comments and encouragement.
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